Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
31628-1-PBS targets VLGR1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34635 Product name: Recombinant human ADGRV1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2269-2812 aa of NM_032119 Sequence: ESIIVSLVYTEGGSRILPSSDTVRVNILANDNVAGIVSFQTASRSVIGHEGEILQFHVIRTFPGRGNVTVNWKIIGQNLELNFANFSGQLFFPEGSLNTTLFVHLLDDNIPEEKEVYQVILYDVRTQGVPPAGIALLDAQGYAA 相同性解析による交差性が予測される生物種 |
| Full Name | G protein-coupled receptor 98 |
| Calculated molecular weight | 693 kDa |
| Observed molecular weight | 217 kDa |
| GenBank accession number | NM_032119 |
| Gene Symbol | GPR98 |
| Gene ID (NCBI) | 84059 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8WXG9 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
VLGR1 (very large G protein-coupled receptor-1), also known as ADGRV1, GPR98, and MASS, has a molecular weight of up to 700 kDa, making it the largest member of the 33 adhesive G protein-coupled receptors (ADGRs), a unique subfamily of the GPCR superfamily. The full-length VLGR1 encompasses ∼6300 amino acids and thus is difficult to analyze by electrophoresis. Vlgr1 is a core component of ankle-link complex in inner ear hair cells. Knock-out and mutation mouse models show that loss of Vlgr1 function leads to abnormal stereociliary development and hearing loss, indicating crucial roles of Vlgr1 in hearing transduction or auditory system development (PMID: 23180093). Western blot analysis detected VLGR1a isoform at an apparent molecular mass of 217 kDa .