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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VLDLR Recombinant antibody (85097-1-RR)

    WB analysis using 85097-1-RR (same clone as 85097-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85097-1-RR (VLDLR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85097-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of THP-1 cells using VLDLR Recombinant antibody (85097-1-RR)

    FC experiment of THP-1 using 85097-1-RR (same clone as 85097-1-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug VLDLR Recombinant Antibody (85097-1-RR, Clone:242385H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85097-1-PBS in a different storage buffer formulation.

VLDLR Recombinant monoclonal antibody, PBS Only

VLDLR Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 85097-1-PBS
Clone No.242385H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

Very low-density lipoprotein receptor, VLDL receptor, VLDL-R

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85097-1-PBS targets VLDLR in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name very low density lipoprotein receptor
Calculated molecular weight 96 kDa
Observed molecular weight120 kDa
GenBank accession numberNM_003383
Gene Symbol VLDLR
Gene ID (NCBI) 7436
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP98155
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The very low-density lipoprotein receptor (VLDLR) is a member of the LDL receptor family with a complex molecular weight profile due to post-translational modifications. The calculated molecular weight of the core protein is approximately 84-86 kDa, however, VLDLR undergoes extensive glycosylation during processing, resulting in higher apparent molecular weights observed in SDS-PAGE analyses. Multiple studies report that glycosylated VLDLR migrates as broad bands between 90-180 kDa under reducing conditions, with the most commonly observed forms at approximately 96 kDa (core glycosylated form), 115-135 kDa (mature fully glycosylated form), and 150-180 kDa (hyperglycosylated or dimeric forms).