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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VLDLR Polyclonal antibody (19493-1-AP)

    WB analysis using 19493-1-AP (same clone as 19493-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19493-1-AP (VLDLR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19493-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VLDLR Polyclonal antibody (19493-1-AP)

    IHC staining of human lung cancer using 19493-1-AP (same clone as 19493-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19493-1-AP (VLDLR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19493-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VLDLR Polyclonal antibody (19493-1-AP)

    IHC staining of human lung cancer using 19493-1-AP (same clone as 19493-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19493-1-AP (VLDLR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19493-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VLDLR Polyclonal antibody (19493-1-AP)

    IF Staining of HeLa using 19493-1-AP (same clone as 19493-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VLDLR antibody (19493-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19493-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

VLDLR Polyclonal antibody, PBS Only

VLDLR Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19493-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Very low-density lipoprotein receptor, VLDL receptor, VLDL-R

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19493-1-PBS targets VLDLR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name very low density lipoprotein receptor
Calculated molecular weight 96 kDa
Observed molecular weight 96 kDa
GenBank accession numberNM_003383
Gene Symbol VLDLR
Gene ID (NCBI) 7436
RRIDAB_10644342
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP98155
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VLDLR binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. VLDLR binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation (cytoplasmic domain of low-density lipoprotein receptor). For glycosylated, the MW of VLDLR migrates to ~160kd.

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