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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VISTA Polyclonal antibody (31619-1-AP)

    WB analysis using 31619-1-AP (same clone as 31619-1-PBS)

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 31619-1-AP (VISTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31619-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VISTA Polyclonal antibody (31619-1-AP)

    IHC staining of human tonsillitis using 31619-1-AP (same clone as 31619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31619-1-AP (VISTA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31619-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VISTA Polyclonal antibody (31619-1-AP)

    IHC staining of human tonsillitis using 31619-1-AP (same clone as 31619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31619-1-AP (VISTA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31619-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using VISTA Polyclonal antibody (31619-1-AP)

    IF Staining of human tonsillitis using 31619-1-AP (same clone as 31619-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (31619-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31619-1-PBS in a different storage buffer formulation.

VISTA Polyclonal antibody, PBS Only

VISTA Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 31619-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

C10orf54, B7-H5, Sisp-1, SISP1, PP2135

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31619-1-PBS targets VISTA in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 54
Calculated molecular weight 311 aa, 34 kDa
Observed molecular weight55 kDa
GenBank accession numberBC020568
Gene Symbol VISTA
Gene ID (NCBI) 64115
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9H7M9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.

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