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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse small intestine tissue using VIPR1 Polyclonal antibody (14878-1-AP)

    WB analysis of mouse small intestine using 14878-1-AP (same clone as 14878-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 14878-1-AP (VIPR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14878-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using VIPR1 Polyclonal antibody (14878-1-AP)

    IHC staining of human kidney using 14878-1-AP (same clone as 14878-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14878-1-AP (VIPR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14878-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using VIPR1 Polyclonal antibody (14878-1-AP)

    IHC staining of human kidney using 14878-1-AP (same clone as 14878-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14878-1-AP (VIPR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14878-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VIPR1 Polyclonal antibody (14878-1-AP)

    IHC staining of mouse brain using 14878-1-AP (same clone as 14878-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14878-1-AP (VIPR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14878-1-PBS in a different storage buffer formulation.

VIPR1 Polyclonal antibody, PBS Only

VIPR1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14878-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14878-1-PBS targets VIPR1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6650

Product name: Recombinant human VIPR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 30-146 aa of BC064424

Sequence: AARLQEECDYVQMIEVQHKQCLEEAQLENETIGCSKMWDNLTCWPATPRGQVVVLACPLIFKLFSSIQGRNVSRSCTDEGWTHLEPGPYPIACGLDDKAASLDEQQTMFYGSVKTGY

相同性解析による交差性が予測される生物種
Full Name vasoactive intestinal peptide receptor 1
Calculated molecular weight 47 kDa
Observed molecular weight50 kDa
GenBank accession numberBC064424
Gene Symbol VIPR1
Gene ID (NCBI) 7433
RRIDAB_2878089
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP32241
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VIPR1(vasoactive intestinal polypeptide receptor 1) is a class B GPCR expressed predominantly in lung, small intestine, thymus, and brain. VIPR1 and its endogenous agonist VIP (28 AA polypeptide) have been reported to be overexpressed in several tumors (PMID: 22365758).

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