Validation Data Gallery
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Tested Applications
Positive WB detected in | A549 cells, A549 |
Positive IHC detected in | Lung cancer tissue, human lung cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
65039-1-Ig targets VIM in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Recombinant Protein 相同性解析による交差性が予測される生物種 |
Full Name | vimentin |
Calculated molecular weight | 54 kDa |
GenBank accession number | BC066956 |
Gene Symbol | VIM |
Gene ID (NCBI) | 7431 |
ENSEMBL Gene ID | ENSG00000026025 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Other |
UNIPROT ID | P08670 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |