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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VGLL1 Polyclonal antibody (10124-2-AP)

    WB analysis using 10124-2-AP (same clone as 10124-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10124-2-AP (VGLL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10124-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using VGLL1 Polyclonal antibody (10124-2-AP)

    WB analysis of rat brain using 10124-2-AP (same clone as 10124-2-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 10124-2-AP (VGLL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10124-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using VGLL1 Polyclonal antibody (10124-2-AP)

    WB analysis of human brain using 10124-2-AP (same clone as 10124-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 10124-2-AP (VGLL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10124-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using VGLL1 Polyclonal antibody (10124-2-AP)

    IHC staining of human prostate cancer using 10124-2-AP (same clone as 10124-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10124-2-AP (VGLL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10124-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

VGLL1 Polyclonal antibody, PBS Only

VGLL1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10124-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10124-2-PBS targets VGLL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0169

Product name: Recombinant human VGLL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 38-255 aa of BC000045

Sequence: SSVVDEHFSRALSNIKSPQELTPSSQSEGVMLKNDDSMSPNQWRYSSPWTKPQPEVPVTNRAANCNLHVPGPMAVNQFSPSLARRASVRPGELWHFSSLAGTSSLEPGYSHPFPARHLVPEPQPDGKREPLLSLLQQDRCLARPQESAARENGNPGQIAGSTGLLFNLPPGSVHYKKLYVSRGSASTSLPNETLSELETPGKYSLTPPNHWGHPHRYL

相同性解析による交差性が予測される生物種
Full Name vestigial like 1 (Drosophila)
Calculated molecular weight 29 kDa
Observed molecular weight 26-29 kDa
GenBank accession numberBC000045
Gene Symbol VGLL1
Gene ID (NCBI) 51442
RRIDAB_2218174
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99990
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VGLL1 is a specific coactivator for the mammalian TEFs. It can bind proteins of the TEA domain family of transcription factors. Vgll1-TEFs complex upregulates the expression of IGFBP-5, a proliferation-promoting gene, and facilitates anchorage-independent cell proliferation. The calculated molecular weight of VGLL1 is 29 kDa, and the observed molecular weight is 26-29 kDa(PMID:31816819,33087697).

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