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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VEZT Polyclonal antibody (17684-1-AP)

    WB analysis using 17684-1-AP (same clone as 17684-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 17684-1-AP (VEZT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using VEZT Polyclonal antibody (17684-1-AP)

    WB analysis of human brain using 17684-1-AP (same clone as 17684-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 17684-1-AP (VEZT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using VEZT Polyclonal antibody (17684-1-AP)

    WB analysis of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17684-1-AP (VEZT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using VEZT Polyclonal antibody (17684-1-AP)

    IP experiment of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

    IP result of anti-VEZT (IP:17684-1-AP, 4ug; Detection:17684-1-AP 1:500) with mouse brain tissue lysate 4400ug. This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VEZT Polyclonal antibody (17684-1-AP)

    IHC staining of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17684-1-AP (VEZT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VEZT Polyclonal antibody (17684-1-AP)

    IHC staining of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17684-1-AP (VEZT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

VEZT Polyclonal antibody, PBS Only

VEZT Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17684-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17684-1-PBS targets VEZT in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11786

Product name: Recombinant human VEZT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 382-731 aa of BC064939

Sequence: VRSLQLHLKALLNEVIILEDELEKLVCTKETQELVSEAYPILEQKLKLIQPHVQASNNCWEEAISQVDKLLRRNTDKKGKPEIACENPHCTVVPLKQPTLHIADKDPIPEEQELEAYVDDIDIDSDFRKDDFYYLSQEDKERQKREHEESKRVLQELKSVLGFKASEAERQKWKQLLFSDHAVLKSLSPVDPVEPISNSEPSMNSDMGKVSKNDTEEESNKSATTDNEISRTEYLCENALEGKNKDNSSNEVFPQGAEERMCYQCESEDEPQADGSGLTTAPPTPRDSLQPSIKQRLARLQLSPDFTFTAGLAAEVAARSLSFTTMQEQTFGDEEEEQIIEENKNEIEEK

相同性解析による交差性が予測される生物種
Full Name vezatin, adherens junctions transmembrane protein
Calculated molecular weight 731 aa, 83 kDa
Observed molecular weight 89 kDa, 125 kDa
GenBank accession numberBC064939
Gene Symbol VEZT
Gene ID (NCBI) 55591
RRIDAB_2213139
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBM0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Vezatin (VEZT) is an adherens junctions transmembrane protein which identified as a putative tumor suppressor. In case of Listeria infection, it promotes bacterial internalization by participating in myosin VIIa recruitment to the entry site. There're two isoforms with calculated MW 89 kDa and 65 kDa. With modification or glycosylation, the MW in Western blot detection will be presented as ~125 kDa and ~89 kDa (PMID: 11080149) or 65-71 kDa and 88-100 kDa (PMID: 20049712). There're some isoforms with MW less than 70 kDa.

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