Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 22601-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 22601-1-AP (VEGFC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 22601-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22601-1-AP (VEGFC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 22601-1-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22601-1-AP (VEGFC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 22601-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22601-1-AP (VEGFC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 22601-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22601-1-AP (VEGFC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 22601-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22601-1-AP (VEGFC Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human lung cancer using 22601-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22601-1-AP (VEGFC Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human lung cancer using 22601-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22601-1-AP (VEGFC Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human lung cancer using 22601-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22601-1-AP (VEGFC Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of RAW 264.7 using 22601-1-AP
Immunofluorescent analysis of RAW 264.7 cells using 22601-1-AP (VEGFC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

VEGF-C Polyclonal antibody

VEGF-C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 22601-1-AP

Publications(44)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

VEGFC, VEGF C, Vascular endothelial growth factor-related protein, vascular endothelial growth factor C

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (44)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF-7 cells, RAW 264.7 cells, HCT 116 cells, SW480 cells
Positive IHC detected in	human prostate cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	RAW 264.7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 32 publications below
IHC	See 13 publications below
IF	See 8 publications below

Product Information

22601-1-AP targets VEGF-C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18182 Product name: Recombinant human VEGFC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 179-228 aa of BC035212 Sequence: SYLSKTLFEITVPLSQGPKPVTISFANHTSCRCMSKLDVYRQVHSIIRRS 相同性解析による交差性が予測される生物種
Full Name	vascular endothelial growth factor C
Calculated molecular weight	419 aa, 47 kDa
Observed molecular weight	47 kDa
GenBank accession number	BC035212
Gene Symbol	VEGFC
Gene ID (NCBI)	7424
RRID	AB_2879132
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49767
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VEGFC is a member of the platelet-derived growth factor / vascular endothelial growth factor (PDGF/VEGF) family. The main function of VEGFC is to promote the growth of lymphatic vessels (lymphangiogenesis). It acts on lymphatic endothelial cells (LECs) primarily via its receptor VEGFR-3 promoting survival, growth, and migration.

Protocols

Product Specific Protocols
IF protocol for VEGF-C antibody 22601-1-AP	Download protocol
IHC protocol for VEGF-C antibody 22601-1-AP	Download protocol
WB protocol for VEGF-C antibody 22601-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cancer Cell Single-cell multi-stage spatial evolutional map of esophageal carcinogenesis Authors - Jiang Chang View Article
mouse	WB,IHC	J Extracell Vesicles DUSP2 regulates extracellular vesicle-VEGF-C secretion and pancreatic cancer early dissemination. Authors - Chu-An Wang View Article
mouse	WB	Clin Transl Med VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis. Authors - Qiu-Yue Lin View Article
human	WB, IHC, IF	Cancer Lett The Hippo-TAZ axis mediates vascular endothelial growth factor C in glioblastoma-derived exosomes to promote angiogenesis. Authors - Zhengmin Wang View Article
mouse	WB	Oxid Med Cell Longev Angiotensin II Induces Cardiac Edema and Hypertrophic Remodeling through Lymphatic-Dependent Mechanisms. Authors - Jie Bai View Article
mouse	IHC	Front Pharmacol Fenofibrate suppresses corneal neovascularization by regulating lipid metabolism through PPARα signaling pathway Authors - Tong Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 22601-1-AP

WB analysis using 22601-1-AP

WB analysis of RAW 264.7 using 22601-1-AP

IHC staining of human prostate cancer using 22601-1-AP

IHC staining of human prostate cancer using 22601-1-AP

IHC staining of human lung cancer using 22601-1-AP

IHC staining of human lung cancer using 22601-1-AP

IHC staining of human lung cancer using 22601-1-AP

IHC staining of human lung cancer using 22601-1-AP

IF Staining of RAW 264.7 using 22601-1-AP

VEGF-C Polyclonal antibody

VEGF-C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Single-cell multi-stage spatial evolutional map of esophageal carcinogenesis

DUSP2 regulates extracellular vesicle-VEGF-C secretion and pancreatic cancer early dissemination.

VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.

The Hippo-TAZ axis mediates vascular endothelial growth factor C in glioblastoma-derived exosomes to promote angiogenesis.

Angiotensin II Induces Cardiac Edema and Hypertrophic Remodeling through Lymphatic-Dependent Mechanisms.

Fenofibrate suppresses corneal neovascularization by regulating lipid metabolism through PPARα signaling pathway