Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IHC | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
26381-1-AP targets VEGFA in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VEGFA fusion protein Ag24740 相同性解析による交差性が予測される生物種 |
| Full Name | vascular endothelial growth factor A |
| Calculated molecular weight | 412 aa, 46 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC065522 |
| Gene Symbol | VEGFA |
| Gene ID (NCBI) | 7422 |
| RRID | AB_2880496 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15692 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VEGFA antibody 26381-1-AP | Download protocol |
| IHC protocol for VEGFA antibody 26381-1-AP | Download protocol |
| FC protocol for VEGFA antibody 26381-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Antioxidants (Basel) Suppression of NRF2 Activity by HIF-1α Promotes Fibrosis after Ischemic Acute Kidney Injury | ||
J Cell Mol Med Piezo type mechanosensitive ion channel component 1 facilitates gastric cancer omentum metastasis. | ||
J Stroke Cerebrovasc Dis LncRNA SNHG12 regulated by WTAP aggravated the oxygen-glucose deprivation/reperfusion-induced injury in bEnd.3 cell | ||
Oncol Lett Mitochondrial calcium uniporter promotes cell proliferation and migration in esophageal cancer. | ||
Cell Death Discov Circ_0030998 promotes tumor proliferation and angiogenesis by sponging miR-567 to regulate VEGFA in colorectal cancer. | ||
Discov Oncol CCL28 promotes progression of hepatocellular carcinoma through PDGFD-regulated MMP9 and VEGFA pathways |