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  • Featured Product
  • KD/KO Validated

VCP Monoclonal antibody

VCP Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ChIP, ELISA

Conjugate

Unconjugated

CloneNo.

2A4B10

Cat no : 60316-1-Ig

Synonyms

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP

Validation Data Gallery

Applications:
  • WB analysis of U2OS using 60316-1-Ig

    WB analysis of U2OS using 60316-1-Ig

    U2OS (WT and VCP KO) lysates prepared with RIPA buffer, 10μg protein loaded. 60316-1-Ig incubated at 1:15000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

  • WB analysis of HeLa using 60316-1-Ig

    WB analysis of HeLa using 60316-1-Ig

    WB result of VCP antibody (60316-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VCP transfected HeLa cells.

  • WB analysis of RAW 264.7 using 60316-1-Ig

    WB analysis of RAW 264.7 using 60316-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60316-1-Ig (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 60316-1-Ig

    WB analysis of SH-SY5Y using 60316-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60316-1-Ig (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 60316-1-Ig

    IHC staining of human gliomas using 60316-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60316-1-Ig (VCP Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with VCP antibody included
  • IHC staining of human gliomas using 60316-1-Ig

    IHC staining of human gliomas using 60316-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60316-1-Ig (VCP Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with VCP antibody included
  • IF Staining of RAW 264.7 using 60316-1-Ig

    IF Staining of RAW 264.7 using 60316-1-Ig

    Immunofluorescent analysis of RAW 264.7 cells using 60316-1-Ig (VCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

  • IF Staining of HeLa using 60316-1-Ig

    IF Staining of HeLa using 60316-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VCP antibody (60316-1-Ig, Clone: 2A4B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of SH-SY5Y using 60316-1-Ig

    IF Staining of SH-SY5Y using 60316-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 60316-1-Ig(VCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HL-60 using 60316-1-Ig

    FC experiment of HL-60 using 60316-1-Ig

    1X10^6 HL-60 cells were intracellularly stained with 0.4 ug Anti-Human VCP (60316-1-Ig, Clone:2A4B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inRAW 264.7 cells, WT and VCP KO U2OS cells, SH-SY5Y cells, HeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells, HeLa cells, SH-SY5Y cells
Positive FC detected inHL-60 cells
Planning an IHC experiment? We recommend our IHCeasy VCP Ready-To-Use IHC Kit. VCP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

60316-1-Ig targets VCP in WB, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VCP fusion protein Ag1002 相同性解析による交差性が予測される生物種
Full Name valosin-containing protein
Calculated molecular weight 89 kDa
Observed molecular weight 89 kDa
GenBank accession numberBC007562
Gene symbol VCP
Gene ID (NCBI) 7415
RRIDAB_2881427
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

Protocols

Product Specific Protocols
WB protocol for VCP antibody 60316-1-IgDownload protocol
IHC protocol for VCP antibody 60316-1-IgDownload protocol
IF protocol for VCP antibody 60316-1-IgDownload protocol
FC protocol for VCP antibody 60316-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Brain

Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.

Authors - Janja Božič
  • KD Validated
View Article
humanWB

Acta Pharmacol Sin

Gossypol, a novel modulator of VCP, induces autophagic degradation of mutant huntingtin by promoting the formation of VCP/p97-LC3-mHTT complex.

Authors - Xiao-Jing Li
View Article
humanWB,ChIP

Biochim Biophys Acta Gene Regul Mech

GLI1, a novel target of the ER stress regulator p97/VCP, promotes ATF6f-mediated activation of XBP1

Authors - Luciana L Almada
View Article
humanWB,IF

Research (Wash D C)

SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage

Authors - Fengli Wang
View Article