Validation Data Gallery

Filter: AllWBIHC

WB analysis using 11444-1-AP (same clone as 11444-1-PBS)
Untreated or TNF-a-treated HUVEC cells were subjected to SDS PAGE followed by western blot with 11444-1-AP (VCAM-1/CD106 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11444-1-AP (VCAM-1/CD106 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11444-1-AP (VCAM-1/CD106 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

VCAM-1/CD106 Polyclonal antibody, PBS Only

VCAM-1/CD106 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11444-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

VCAM1, VCAM-1, CD106, INCAM 100, INCAM-100

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11444-1-PBS targets VCAM-1/CD106 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1993 Product name: Recombinant human VCAM-1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 341-739 aa of BC017276 Sequence: ESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPELLVLYFASSLIIPAIGMIIYFARKANMKGSYSLVEAQKSKV 相同性解析による交差性が予測される生物種
Full Name	vascular cell adhesion molecule 1
Calculated molecular weight	739 aa, 81 kDa
Observed molecular weight	110 kDa
GenBank accession number	BC017276
Gene Symbol	VCAM-1
Gene ID (NCBI)	7412
RRID	AB_2214232
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P19320
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11444-1-AP (same clone as 11444-1-PBS)

IHC staining of human tonsillitis using 11444-1-AP (same clone as 11444-1-PBS)

IHC staining of human tonsillitis using 11444-1-AP (same clone as 11444-1-PBS)

IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)

IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)

IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)

IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)

IHC staining of human placenta using 11444-1-AP (same clone as 11444-1-PBS)

IHC staining of human placenta using 11444-1-AP (same clone as 11444-1-PBS)

IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)

IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)

IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)

IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)

IHC staining of human lung cancer using 11444-1-AP (same clone as 11444-1-PBS)

IHC staining of human lung cancer using 11444-1-AP (same clone as 11444-1-PBS)

VCAM-1/CD106 Polyclonal antibody, PBS Only

VCAM-1/CD106 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information