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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VAV3 Polyclonal antibody (30291-1-AP)

    WB analysis using 30291-1-AP (same clone as 30291-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30291-1-AP (VAV3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30291-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using VAV3 Polyclonal antibody (30291-1-AP)

    IP experiment of Jurkat using 30291-1-AP (same clone as 30291-1-PBS)

    IP result of anti-VAV3 (IP:30291-1-AP, 4ug; Detection:30291-1-AP 1:8000) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 30291-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using VAV3 Polyclonal antibody (30291-1-AP)

    IHC staining of human kidney using 30291-1-AP (same clone as 30291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 30291-1-AP (VAV3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30291-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using VAV3 Polyclonal antibody (30291-1-AP)

    IHC staining of mouse kidney using 30291-1-AP (same clone as 30291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30291-1-AP (VAV3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30291-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VAV3 Polyclonal antibody (30291-1-AP)

    IHC staining of human colon cancer using 30291-1-AP (same clone as 30291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30291-1-AP (VAV3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30291-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VAV3 Polyclonal antibody (30291-1-AP)

    IF Staining of HeLa using 30291-1-AP (same clone as 30291-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VAV3 antibody (30291-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30291-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using VAV3 Polyclonal antibody (30291-1-AP)

    IF Staining of Jurkat using 30291-1-AP (same clone as 30291-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using VAV3 antibody (30291-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30291-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using VAV3 Polyclonal antibody (30291-1-AP)

    FC experiment of Jurkat using 30291-1-AP (same clone as 30291-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human VAV3 (30291-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 30291-1-PBS in a different storage buffer formulation.

VAV3 Polyclonal antibody, PBS Only

VAV3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 30291-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Guanine nucleotide exchange factor VAV3, VAV 3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30291-1-PBS targets VAV3 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag33022

Product name: Recombinant human VAV3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 546-847 aa of NM_006113

Sequence: KCGARAHKECLGRVDNCGRVNSGEQGTLKLPEKRTNGLRRTPKQVDPGLPKMQVIRNYSGTPPPALHEGPPLQLQAGDTVELLKGDAHSLFWQGRNLASGEVGFFPSDAVKPCPCVPKPVDYSCQPWYAGAMERLQAETELINRVNSTYLVRHRTKESGEYAISIKYNNEAKHIKILTRDGFFHIAENRKFKSLMELVEYYKHHSLKEGFRTLDTTLQFPYKEPEHSAGQRGNRAGNSLLSPKVLGIAIARYDFCARDMRELSLLKGDVVKIYTKMSANGWWRGEVNGRVGWFPSTYVEEDE

相同性解析による交差性が予測される生物種
Full Name vav 3 guanine nucleotide exchange factor
Calculated molecular weight98kd
Observed molecular weight 98 kDa
GenBank accession numberNM_006113
Gene Symbol VAV3
Gene ID (NCBI) 10451
RRIDAB_3086288
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKW4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Vav3, a member of Dbl family which is also the activator of GTP kinase of Rho family, can activate the expression of RhoA, Racl and Cdc42 (PMID:28285969). Vav3 is associated with tumor growth, apoptosis, invasion, metastasis and angiogenesis (PMID:28285969). Vav3 is a multiple function protein with both signaling molecule and coactivator activities. Vav3 oncogene is overexpressed in androgen-independent prostate cancer cells relative to their androgen-dependent counterparts and contribute to development of hormone refractory prostate cancer (PMID:21455584).

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