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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using VAV2 Monoclonal antibody (67108-1-Ig)

    WB analysis of fetal human brain using 67108-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using VAV2 Monoclonal antibody (67108-1-Ig)

    WB analysis of pig brain using 67108-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using VAV2 Monoclonal antibody (67108-1-Ig)

    WB analysis of rat brain using 67108-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using VAV2 Monoclonal antibody (67108-1-Ig)

    WB analysis of mouse brain using 67108-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using VAV2 Monoclonal antibody (67108-1-Ig)

    WB analysis of A431 using 67108-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using VAV2 Monoclonal antibody (67108-1-Ig)

    WB analysis of HeLa using 67108-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using VAV2 Monoclonal antibody (67108-1-Ig)

    WB analysis of HEK-293 using 67108-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using VAV2 Monoclonal antibody (67108-1-Ig)

    IF Staining of A431 using 67108-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using VAV2 antibody (67108-1-Ig, Clone: 3F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using VAV2 Monoclonal antibody (67108-1-Ig)

    IF Staining of HUVEC using 67108-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using VAV2 antibody (67108-1-Ig, Clone: 3F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VAV2 Monoclonal antibody (67108-1-Ig)

    IF Staining of HeLa using 67108-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VAV2 antibody (67108-1-Ig, Clone: 3F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

VAV2 Monoclonal antibody

VAV2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67108-1-Ig
Clone No.3F11B5

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

VAV 2, VAV2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected infetal human brain tissue, A431 cells, HeLa cells, HEK-293 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inA431 cells, HeLa cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67108-1-Ig targets VAV2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, mouse, rat samples.

Tested Reactivity Human, pig, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17988

Product name: Recombinant human VAV2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 98-326 aa of BC132967

Sequence: DLFDVRDFGKVISAVSRLSLHSIAQNKGIRPFPSEETTENDDDVYRSLEELADEHDLGEDIYDCVPCEDGGDDIYEDIIKVEVQQPMKMGMTEDDKRNCCLLEIQETEAKYYRTLEDIEKNYMSPLRLVLSPADMAAVFINLEDLIKVHHSFLRAIDVSVMVGGSTLAKVFLDFKERLLIYGEYCSHMEHAQNTLNQLLASREDFRQKVEECTLKVQDGKFKLQDLLVV

相同性解析による交差性が予測される生物種
Full Name vav 2 guanine nucleotide exchange factor
Calculated molecular weight 839 aa, 97 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC132967
Gene Symbol VAV2
Gene ID (NCBI) 7410
RRIDAB_2882412
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP52735
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for VAV2 antibody 67108-1-IgDownload protocol
WB protocol for VAV2 antibody 67108-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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