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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VAV1 Recombinant monoclonal antibody (86933-2-RR)

    WB analysis using 86933-2-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 86933-2-RR (VAV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using VAV1 Recombinant monoclonal antibody (86933-2-RR)

    IF Staining of Jurkat using 86933-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using VAV1 antibody (86933-2-RR, Clone: 251870A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of Jurkat cells using VAV1 Recombinant monoclonal antibody (86933-2-RR)

    FC experiment of Jurkat using 86933-2-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug VAV1 Recombinant monoclonal antibody (86933-2-RR, Clone:251870A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • Affinity and Kinetic Characterization of 86933-2-RR

    Affinity and Kinetic Characterization of 86933-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86933-2-RR against Human VAV1 were performed. The affinity constant is below 1 pM.

VAV1 Recombinant monoclonal antibody

VAV1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 86933-2-RR
Clone No.251870A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Proto oncogene vav, Proto-oncogene vav, VAV

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, THP-1 cells, K-562 cells, Raji cells
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86933-2-RR targets VAV1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag38665

Product name: Recombinant human VAV1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 442-790 aa of BC013361

Sequence: EQFEMAISNIYPENATANGHDFQMFSFEETTSCKACQMLLRGTFYQGYRCHRCRASAHKECLGRVPPCGRHGQDFPGTMKKDKLHRRAQDKKRNELGLPKMEVFQEYYGLPPPPGAIGPFLRLNPGDIVELTKAEAEQNWWEGRNTSTNEIGWFPCNRVKPYVHGPPQDLSVHLWYAGPMERAGAESILANRSDGTFLVRQRVKDAAEFAISIKYNVEVKHIKIMTAEGLYRITEKKAFRGLTELVEFYQQNSLKDCFKSLDTTLQFPFKEPEKRTISRPAVGSTKYFGTAKARYDFCARDRSELSLKEGDIIKILNKKGQQGWWRGEIYGRVGWFPANYVEEDYSEYC

相同性解析による交差性が予測される生物種
Full Name vav 1 guanine nucleotide exchange factor
Calculated molecular weight 845 aa, 98 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC013361
Gene Symbol VAV1
Gene ID (NCBI) 7409
RRIDAB_3745216
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP15498
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vav proteins mainly act as enzymes that catalyze the activation step of Rho subfamily GTPases during cell signaling. There are three family members:VAV1, VAV2 and VAV3. Vav1 is specifically expressed in the hematopoietic system, whereas Vav2 and Vav3 are more ubiquitously expressed (PMID:14607270). Vav1 is physiologically active as a GDP/GTP nucleotide exchange factor (GEF) in the hematopoietic system (PMID:31654719).

Protocols

Product Specific Protocols
FC protocol for VAV1 antibody 86933-2-RRDownload protocol
IF protocol for VAV1 antibody 86933-2-RRDownload protocol
WB protocol for VAV1 antibody 86933-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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