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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VAT1 Recombinant monoclonal antibody (86847-1-RR)

    WB analysis using 86847-1-RR (same clone as 86847-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86847-1-RR (VAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86847-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using VAT1 Recombinant monoclonal antibody (86847-1-RR)

    WB analysis using 86847-1-RR (same clone as 86847-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86847-1-RR (VAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86847-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VAT1 Recombinant monoclonal antibody (86847-1-RR)

    IHC staining of mouse brain using 86847-1-RR (same clone as 86847-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86847-1-RR (VAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86847-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VAT1 Recombinant monoclonal antibody (86847-1-RR)

    IHC staining of mouse brain using 86847-1-RR (same clone as 86847-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86847-1-RR (VAT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86847-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using VAT1 Recombinant monoclonal antibody (86847-1-RR)

    IF Staining of SH-SY5Y using 86847-1-RR (same clone as 86847-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using VAT1 antibody (86847-1-RR, Clone: 251841D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86847-1-PBS in a different storage buffer formulation.

VAT1 Recombinant monoclonal antibody, PBS Only

VAT1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86847-1-PBS
Clone No.251841D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:1.-.-.-, Synaptic vesicle membrane protein VAT-1 homolog, VATI

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86847-1-PBS targets VAT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag16652

Product name: Recombinant human VAT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-393 aa of BC015041

Sequence: MSDEREVAEAATGEDASSPPPKTEAASDPQHPAASEGAAAAAASPPLLRCLVLTGFGGYDKVKLQSRPAAPPAPGPGQLTLRLRACGLNFADLMARQGLYDRLPPLPVTPGMEGAGVVIAVGEGVSDRKAGDRVMVLNRSGMWQEEVTVPSVQTFLIPEAMTFEEAAALLVNYITAYMVLFDFGNLQPGHSVLVHMAAGGVGMAAVQLCRTVENVTVFGTASASKHEALKENGVTHPIDYHTTDYVDEIKKISPKGVDIVMDPLGGSDTAKGYNLLKPMGKVVTYGMANLLTGPKRNLMALARTWWNQFSVTALQLLQANRAVCGFHLGYLDGEVELVSGVVARLLALYNQGHIKPHIDSVWPFEKVADAMKQMQEKKNVGKVLLVPGPEKEN

相同性解析による交差性が予測される生物種
Full Name vesicle amine transport protein 1 homolog (T. californica)
Calculated molecular weight 42 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC015041
Gene Symbol VAT1
Gene ID (NCBI) 10493
RRIDAB_3745159
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ99536
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Vesicle amine transport protein 1 (VAT1) is expressed in neurons and neuroendocrine cells, where it participates in vesicular monoamine uptake and in the docking/fusion steps of neurotransmitter release. Beyond the nervous system, VAT1 binds anionic phospholipids (phosphatidic acid and phosphatidylserine) and catalyses their inter-membrane transfer, thereby integrating lipid metabolism with vesicle trafficking. Recent tumour-biology studies have revealed that VAT1 is frequently up-regulated in aggressive gliomas and gastric cancers; its abundance correlates with enhanced cell migration, radioresistance and an immunosuppressive micro-environment. (PMID: 39832459,PMID: 31524269)

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