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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VASH2 Polyclonal antibody (13303-1-AP)

    WB analysis using 13303-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13303-1-AP (VASH2 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VASH2 Polyclonal antibody (13303-1-AP)

    WB analysis using 13303-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13303-1-AP (VASH2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using VASH2 Polyclonal antibody (13303-1-AP)

    WB analysis of L02 using 13303-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 13303-1-AP (VASH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using VASH2 Polyclonal antibody (13303-1-AP)

    IHC staining of human stomach cancer using 13303-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13303-1-AP (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using VASH2 Polyclonal antibody (13303-1-AP)

    IHC staining of human stomach cancer using 13303-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13303-1-AP (VASH2 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VASH2 Polyclonal antibody (13303-1-AP)

    IHC staining of human liver cancer using 13303-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13303-1-AP (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VASH2 Polyclonal antibody (13303-1-AP)

    IHC staining of human liver cancer using 13303-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13303-1-AP (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using VASH2 Polyclonal antibody (13303-1-AP)

    IHC staining of human stomach cancer using 13303-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13303-1-AP (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using VASH2 Polyclonal antibody (13303-1-AP)

    IF Staining of A549 using 13303-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using VASH2 antibody (13303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

VASH2 Polyclonal antibody

VASH2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13303-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Vasohibin-like protein, Vasohibin-2, VASHL, Tubulinyl-Tyr carboxypeptidase 2, EC:3.4.17.17

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHUVEC cells, BxPC-3 cells, L02 cells, SW 1990 cells, PANC-1 cells, PC-12 cells
Positive IHC detected inhuman stomach cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13303-1-AP targets VASH2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VASH2 fusion protein Ag4121 相同性解析による交差性が予測される生物種
Full Name vasohibin 2
Calculated molecular weight 355 aa, 40 kDa
Observed molecular weight30-34 kDa
GenBank accession numberBC028194
Gene Symbol VASH2
Gene ID (NCBI) 79805
RRIDAB_10642836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86V25
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VASH2, also named as vasohibin 2, is an important pro-angiogenesis factor in solid tumor. It has been reported that VASH2 is expressed in mononuclear cells mobilized from bone marrow to promote angiogenesis. VASH2 also plays a key role in axon formation. VASH2 is localized as a nuclear type(with 311 amino acid residues) and cytoplasmic type (with 355 amino acid residues and low abundance). Cytoplasmic VASH2 is associated with carcinoma angiogenesis, while nuclear VASH2 may be associated with cell proliferation. This antibody recognizes both the nuclear and cytoplasmic isoforms.(PMID:23615928; 19204325; 31235911; 26177649)

Protocols

Product Specific Protocols
WB protocol for VASH2 antibody 13303-1-APDownload protocol
IHC protocol for VASH2 antibody 13303-1-APDownload protocol
IF protocol for VASH2 antibody 13303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Basic Res Cardiol

Extracellular stiffness induces contractile dysfunction in adult cardiomyocytes via cell-autonomous and microtubule-dependent mechanisms

Authors - Alexia Vite
View Article