Validation Data Gallery
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Tested Applications
| Positive WB detected in | HL-60 cells, HeLa cells, HepG2 cells, L02 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IP | See 2 publications below |
Product Information
15931-1-AP targets ValRS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8778 Product name: Recombinant human VARS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 916-1264 aa of BC012808 Sequence: ECGTDALRFGLCAYMSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSQPGGHESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRMPQAPPSLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASDRCSIHLQLQGLVDPARELGKLQAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML 相同性解析による交差性が予測される生物種 |
| Full Name | valyl-tRNA synthetase |
| Calculated molecular weight | 1264 aa, 140 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | BC012808 |
| Gene Symbol | ValRS |
| Gene ID (NCBI) | 7407 |
| RRID | AB_2213252 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P26640 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ValRS antibody 15931-1-AP | Download protocol |
| IP protocol for ValRS antibody 15931-1-AP | Download protocol |
| WB protocol for ValRS antibody 15931-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation | ||
Autoimmun Rev Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase | ||
Ann Rheum Dis Mass spectrometry-based identification of new anti-Ly and known antisynthetase autoantibodies |
