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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VAPA Monoclonal antibody (67845-1-Ig)

    WB analysis using 67845-1-Ig (same clone as 67845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67845-1-Ig (VAPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VAPA Monoclonal antibody (67845-1-Ig)

    IHC staining of human liver cancer using 67845-1-Ig (same clone as 67845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VAPA Monoclonal antibody (67845-1-Ig)

    IHC staining of human liver cancer using 67845-1-Ig (same clone as 67845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VAPA Monoclonal antibody (67845-1-Ig)

    IHC staining of human colon cancer using 67845-1-Ig (same clone as 67845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VAPA Monoclonal antibody (67845-1-Ig)

    IHC staining of human colon cancer using 67845-1-Ig (same clone as 67845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

VAPA Monoclonal antibody, PBS Only

VAPA Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67845-1-PBS
Clone No.1F10F9

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Synonyms

33 kDa VAMP associated protein, hVAP 33, VAMP A, VAMP associated protein A, VAP 33, VAP A, VAP33, VAPA

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67845-1-PBS targets VAPA in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7819

Product name: Recombinant human VAPA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-221 aa of BC002992

Sequence: MASASGAMAKHEQILVLDPPTDLKFKGPFTDVVTTNLKLRNPSDRKVCFKVKTTAPRRYCVRPNSGIIDPGSTVTVSVMLQPFDYDPNEKSKHKFMVQTIFAPPNTSDMEAVWKEAKPDELMDSKLRCVFEMPNENDKLNDMEPSKAVPLNASKQDGPMPKPHSVSLNDTETRKLMEECKRLQGEMMKLSEENRHLRDEGLRLRKVAHSDKPGSTSTASFR

相同性解析による交差性が予測される生物種
Full Name VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Calculated molecular weight 28 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC002992
Gene Symbol VAPA
Gene ID (NCBI) 9218
RRIDAB_2918607
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9P0L0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a 33-kDa, ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.

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