Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 15275-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15275-1-AP
WB result of VAPA antibody (15275-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VAPA transfected HEK-293 cells.
WB analysis using 15275-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 15275-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 15275-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15275-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 15275-1-AP
IP result of anti-VAPA (IP:15275-1-AP, 3ug; Detection:15275-1-AP 1:1000) with HEK-293 cells lysate 3200ug.
IHC staining of human colon cancer using 15275-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15275-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15275-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15275-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15275-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 15275-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 15275-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15275-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPA antibody (15275-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Featured Product
KD/KO Validated

VAPA Polyclonal antibody

VAPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15275-1-AP

Publications(42)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, COIP, ELISA

Synonyms

33 kDa VAMP associated protein, 33 kDa VAMP-associated protein, hVAP 33, VAMP A, VAMP associated protein A

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (42)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse skeletal muscle tissue, human kidney tissue, human brain tissue, HepG2 cells, mouse liver tissue, rat liver tissue, HeLa cells, K-562 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human colon cancer tissue, human liver cancer tissue, mouse brain tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 30 publications below
IHC	See 2 publications below
IF	See 13 publications below
IP	See 3 publications below
CoIP	See 3 publications below

Product Information

15275-1-AP targets VAPA in WB, IHC, IF/ICC, IP, COIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7393 Product name: Recombinant human VAPA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-221 aa of BC002992 Sequence: MASASGAMAKHEQILVLDPPTDLKFKGPFTDVVTTNLKLRNPSDRKVCFKVKTTAPRRYCVRPNSGIIDPGSTVTVSVMLQPFDYDPNEKSKHKFMVQTIFAPPNTSDMEAVWKEAKPDELMDSKLRCVFEMPNENDKLNDMEPSKAVPLNASKQDGPMPKPHSVSLNDTETRKLMEECKRLQGEMMKLSEENRHLRDEGLRLRKVAHSDKPGSTSTASFR 相同性解析による交差性が予測される生物種
Full Name	VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Calculated molecular weight	28 kDa
Observed molecular weight	28-30 kDa
GenBank accession number	BC002992
Gene Symbol	VAPA
Gene ID (NCBI)	9218
RRID	AB_2256991
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9P0L0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.

Protocols

Product Specific Protocols
IF protocol for VAPA antibody 15275-1-AP	Download protocol
IHC protocol for VAPA antibody 15275-1-AP	Download protocol
IP protocol for VAPA antibody 15275-1-AP	Download protocol
WB protocol for VAPA antibody 15275-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB,CoIP	Cell Calcium transients on the ER surface trigger liquid-liquid phase separation of FIP200 to specify autophagosome initiation sites Authors - Qiaoxia Zheng KD Validated View Article
human	WB,IP	Nat Microbiol Toxoplasma effector TgROP1 establishes membrane contact sites with the endoplasmic reticulum during infection. Authors - Chahat Mehra View Article
human	WB	Autophagy USP20 deubiquitinates and stabilizes the reticulophagy receptor RETREG1/FAM134B to drive reticulophagy Authors - Man Zhang View Article
mouse	WB,IP	Nat Commun Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder. Authors - Bryan J. Song View Article
mouse	WB,IHC	Nat Commun Bipolar and schizophrenia risk gene AKAP11 encodes an autophagy receptor coupling the regulation of PKA kinase network homeostasis to synaptic transmission. Authors - You-Kyung Lee View Article
mouse,human	WB	Nat Commun Variants in the WDR44 WD40-repeat domain cause a spectrum of ciliopathy by impairing ciliogenesis initiation Authors - Andrea Accogli View Article

Validation Data Gallery

WB analysis of HEK-293 using 15275-1-AP

WB analysis of HEK-293 using 15275-1-AP

WB analysis using 15275-1-AP

WB analysis of mouse skeletal muscle using 15275-1-AP

WB analysis of human kidney using 15275-1-AP

WB analysis of human brain using 15275-1-AP

IP experiment of HEK-293 using 15275-1-AP

IHC staining of human colon cancer using 15275-1-AP

IHC staining of human colon cancer using 15275-1-AP

IHC staining of mouse brain using 15275-1-AP

IHC staining of human liver cancer using 15275-1-AP

IHC staining of human liver cancer using 15275-1-AP

IHC staining of mouse heart using 15275-1-AP

IHC staining of mouse heart using 15275-1-AP

IF Staining of HepG2 using 15275-1-AP

VAPA Polyclonal antibody

VAPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Calcium transients on the ER surface trigger liquid-liquid phase separation of FIP200 to specify autophagosome initiation sites

Toxoplasma effector TgROP1 establishes membrane contact sites with the endoplasmic reticulum during infection.

USP20 deubiquitinates and stabilizes the reticulophagy receptor RETREG1/FAM134B to drive reticulophagy

Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder.

Bipolar and schizophrenia risk gene AKAP11 encodes an autophagy receptor coupling the regulation of PKA kinase network homeostasis to synaptic transmission.

Variants in the WDR44 WD40-repeat domain cause a spectrum of ciliopathy by impairing ciliogenesis initiation

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