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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using VANGL2 Monoclonal antibody (67273-1-Ig)

    WB analysis of MCF-7 using 67273-1-Ig (same clone as 67273-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67273-1-Ig (VANGL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67273-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using VANGL2 Monoclonal antibody (67273-1-Ig)

    WB analysis of HepG2 using 67273-1-Ig (same clone as 67273-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67273-1-Ig (VANGL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67273-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using VANGL2 Monoclonal antibody (67273-1-Ig)

    WB analysis of HeLa using 67273-1-Ig (same clone as 67273-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67273-1-Ig (VANGL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67273-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

VANGL2 Monoclonal antibody, PBS Only

VANGL2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67273-1-PBS
Clone No.1B2C1

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

LTAP, LPP1, Loop-tail protein 1 homolog, Loop tail protein 1 homolog, KIAA1215

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67273-1-PBS targets VANGL2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag16980

Product name: Recombinant human VANGL2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 268-322 aa of BC103920

Sequence: IQRVAVWILEKYYHDFPVYNPALLNLPKSVLAKKVSGFKVYSLGEENSTNNSTGQ

相同性解析による交差性が予測される生物種
Full Name vang-like 2 (van gogh, Drosophila)
Calculated molecular weight 521 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC103920
Gene Symbol VANGL2
Gene ID (NCBI) 57216
RRIDAB_2882542
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9ULK5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Vangl2 is a key component of the planar cell polarity (PCP) pathway. Vangl2 is tightly associated with the postsynaptic density (PSD) fraction and forms a protein complex with PSD-95 and NMDA receptors. Vangl2 directly binds to the third PDZ domain of PSD-95 via its C-terminal TSV motif. Vangl2 directly binds to N-cadherin.

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