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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VANGL2 Polyclonal antibody (21492-1-AP)

    WB analysis using 21492-1-AP (same clone as 21492-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21492-1-AP (VANGL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using VANGL2 Polyclonal antibody (21492-1-AP)

    WB analysis using 21492-1-AP (same clone as 21492-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21492-1-AP (VANGL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using VANGL2 Polyclonal antibody (21492-1-AP)

    IP experiment of HepG2 using 21492-1-AP (same clone as 21492-1-PBS)

    IP result of anti-VANGL2 (IP:21492-1-AP, 4ug; Detection:21492-1-AP 1:500) with HepG2 cells lysate 1880 ug. This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using VANGL2 Polyclonal antibody (21492-1-AP)

    IHC staining of human stomach using 21492-1-AP (same clone as 21492-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21492-1-AP (VANGL2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using VANGL2 Polyclonal antibody (21492-1-AP)

    IHC staining of human stomach using 21492-1-AP (same clone as 21492-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21492-1-AP (VANGL2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

VANGL2 Polyclonal antibody, PBS Only

VANGL2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 21492-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

KIAA1215, Loop tail protein 1 homolog, Loop-tail protein 1 homolog, LPP1, LTAP

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21492-1-PBS targets VANGL2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15833

Product name: Recombinant human VANGL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 268-322 aa of BC103920

Sequence: IQRVAVWILEKYYHDFPVYNPALLNLPKSVLAKKVSGFKVYSLGEENSTNNSTGQ

相同性解析による交差性が予測される生物種
Full Name vang-like 2 (van gogh, Drosophila)
Calculated molecular weight 521 aa, 60 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC103920
Gene Symbol VANGL2
Gene ID (NCBI) 57216
RRIDAB_11182263
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULK5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Vangl2 is a key component of the planar cell polarity (PCP) pathway. Vangl2 is tightly associated with the postsynaptic density (PSD) fraction and forms a protein complex with PSD-95 and NMDA receptors. Vangl2 directly binds to the third PDZ domain of PSD-95 via its C-terminal TSV motif. Vangl2 directly binds to N-cadherin.

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