Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, THP-1 cells, mouse kidney tissue, rat kidney tissue |
| Positive IP detected in | THP-1 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
RMX00044 targets VAMP8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | vesicle-associated membrane protein 8 (endobrevin) |
| Calculated molecular weight | 11 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC001634 |
| Gene Symbol | VAMP8 |
| Gene ID (NCBI) | 8673 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | N/A |
| UNIPROT ID | Q9BV40 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}} |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VAMP8, also named endobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy by directly controlling the fusion of autophagosomes with the lysosomal membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for VAMP8 antibody RMX00044 | Download protocol |
| IHC protocol for VAMP8 antibody RMX00044 | Download protocol |
| IP protocol for VAMP8 antibody RMX00044 | Download protocol |
| WB protocol for VAMP8 antibody RMX00044 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |