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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VAMP8 Recombinant antibody (85400-4-RR)

    WB analysis using 85400-4-RR (same clone as 85400-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85400-4-RR (VAMP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85400-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VAMP8 Recombinant antibody (85400-4-RR)

    IHC staining of human placenta using 85400-4-RR (same clone as 85400-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85400-4-RR (VAMP8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85400-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VAMP8 Recombinant antibody (85400-4-RR)

    IHC staining of human placenta using 85400-4-RR (same clone as 85400-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85400-4-RR (VAMP8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85400-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using VAMP8 Recombinant antibody (85400-4-RR)

    IF Staining of THP-1 using 85400-4-RR (same clone as 85400-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using VAMP8 antibody (85400-4-RR, Clone: 242830B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85400-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85400-4-RR

    Affinity and Kinetic Characterization of 85400-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85400-4-RR against Human VAMP8 were performed. The affinity constant is 0.631 nM.

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VAMP8 Recombinant antibody, PBS Only

VAMP8 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85400-4-PBS
Clone No.242830B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

VAMP-8, 242830B11, EDB, Endobrevin, VAMP 8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85400-4-PBS targets VAMP8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen VAMP8 fusion protein Ag7903 相同性解析による交差性が予測される生物種
Full Name vesicle-associated membrane protein 8 (endobrevin)
Calculated molecular weight 11 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC001634
Gene Symbol VAMP8
Gene ID (NCBI) 8673
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BV40
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

VAMP8, also named as endobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells.