Validation Data Gallery

Filter: AllWBIHC

WB analysis using 15546-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 15546-1-AP (VAMP8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 15546-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15546-1-AP (VAMP8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of PC-12 using 15546-1-AP
PC-12 cells were subjected to SDS PAGE followed by western blot with 15546-1-AP (VAMP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 15546-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 15546-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse placenta using 15546-1-AP
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse placenta using 15546-1-AP
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VAMP8 Polyclonal antibody

VAMP8 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15546-1-AP

Publications(24)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

EDB, Endobrevin, VAMP 8, VAMP8

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (24)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HL-60 cells, PC-12 cells, THP-1 cells
Positive IHC detected in	human placenta tissue, mouse placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 19 publications below
IHC	See 1 publications below
IF	See 4 publications below
IP	See 2 publications below
CoIP	See 2 publications below

Product Information

15546-1-AP targets VAMP8 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7903 Product name: Recombinant human VAMP8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-81 aa of BC001634 Sequence: MEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVLIC 相同性解析による交差性が予測される生物種
Full Name	vesicle-associated membrane protein 8 (endobrevin)
Calculated molecular weight	11 kDa
Observed molecular weight	15 kDa
GenBank accession number	BC001634
Gene Symbol	VAMP8
Gene ID (NCBI)	8673
RRID	AB_2878150
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BV40
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VAMP8, also named as endobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells.

Protocols

Product Specific Protocols
IHC protocol for VAMP8 antibody 15546-1-AP	Download protocol
WB protocol for VAMP8 antibody 15546-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	J Extracell Vesicles Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion Authors - Chuqi Liu View Article
human	WB	Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes Authors - Wang Zheng View Article
human	WB,IF	Cell Mol Life Sci IGF2BP1-HAX-1 positive feedback loop-mediated HAX-1 overexpression blocks autophagic flux and promotes chemoresistance in nasopharyngeal carcinoma Authors - Siyu Zhang View Article
rat	WB	Arch Toxicol Mystery of methamphetamine-induced autophagosome accumulation in hippocampal neurons: loss of syntaxin 17 in defects of dynein-dynactin driving and autophagosome-late endosome/lysosome fusion. Authors - Huaisha Xu View Article
human	WB	Ecotoxicol Environ Saf Weakened interaction of ATG14 and the SNARE complex blocks autophagosome-lysosome fusion contributes to fluoride-induced developmental neurotoxicity. Authors - Yuanli Zhang View Article
rat	WB	Stem Cell Res Ther Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells. Authors - Zheng Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15546-1-AP

WB analysis using 15546-1-AP

WB analysis of PC-12 using 15546-1-AP

IHC staining of human placenta using 15546-1-AP

IHC staining of human placenta using 15546-1-AP

IHC staining of mouse placenta using 15546-1-AP

IHC staining of mouse placenta using 15546-1-AP

VAMP8 Polyclonal antibody

VAMP8 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

IGF2BP1-HAX-1 positive feedback loop-mediated HAX-1 overexpression blocks autophagic flux and promotes chemoresistance in nasopharyngeal carcinoma

Mystery of methamphetamine-induced autophagosome accumulation in hippocampal neurons: loss of syntaxin 17 in defects of dynein-dynactin driving and autophagosome-late endosome/lysosome fusion.

Weakened interaction of ATG14 and the SNARE complex blocks autophagosome-lysosome fusion contributes to fluoride-induced developmental neurotoxicity.

Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells.