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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VAMP4 Monoclonal antibody (67219-1-Ig)

    WB analysis using 67219-1-Ig (same clone as 67219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67219-1-Ig (VAMP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using VAMP4 Monoclonal antibody (67219-1-Ig)

    IHC staining of rat brain using 67219-1-Ig (same clone as 67219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using VAMP4 Monoclonal antibody (67219-1-Ig)

    IHC staining of rat brain using 67219-1-Ig (same clone as 67219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using VAMP4 Monoclonal antibody (67219-1-Ig)

    IHC staining of mouse cerebellum using 67219-1-Ig (same clone as 67219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using VAMP4 Monoclonal antibody (67219-1-Ig)

    IHC staining of mouse cerebellum using 67219-1-Ig (same clone as 67219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using VAMP4 Monoclonal antibody (67219-1-Ig)

    IF Staining of HEK-293 using 67219-1-Ig (same clone as 67219-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67219-1-Ig (VAMP4 antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

VAMP4 Monoclonal antibody, PBS Only

VAMP4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67219-1-PBS
Clone No.3A6F2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

3A6F2, VAMP-4, Vesicle-associated membrane protein 4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67219-1-PBS targets VAMP4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27854

Product name: Recombinant human VAMP4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-140 aa of BC007019

Sequence: MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLGPSGPRFGPRNDKIKHVQNQVDEVIDVMQENITKVIERGERLDELQDKSESLSDNATAFSNRSKQLRRQMWWRGCKIKAIMALVAAILLLVIIILIVMKYRT

相同性解析による交差性が予測される生物種
Full Name vesicle-associated membrane protein 4
Calculated molecular weight 16 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC007019
Gene Symbol VAMP4
Gene ID (NCBI) 8674
RRIDAB_2882510
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO75379
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.

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