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Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using VAMP3/Cellubrevin Monoclonal antibody (66488-1-Ig)

    WB analysis of pig brain using 66488-1-Ig (same clone as 66488-1-PBS)

    Pig brain, rat brain and mouse brain tissues were subjected to SDS PAGE followed by western blot with 66488-1-Ig (VAMP3/Cellubrevin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using VAMP3/Cellubrevin Monoclonal antibody (66488-1-Ig)

    WB analysis of HEK-293 using 66488-1-Ig (same clone as 66488-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66488-1-Ig (VAMP3/Cellubrevin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using VAMP3/Cellubrevin Monoclonal antibody (66488-1-Ig)

    IF Staining of Neuro-2a using 66488-1-Ig (same clone as 66488-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using VAMP3/Cellubrevin antibody (66488-1-Ig, Clone: 1D10D10 ) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

VAMP3/Cellubrevin Monoclonal antibody, PBS Only

VAMP3/Cellubrevin Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66488-1-PBS
Clone No.1D10D10

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

VAMP3, Synaptobrevin-3, SYB3, Cellubrevin, CEB

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66488-1-PBS targets VAMP3/Cellubrevin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag1156

Product name: Recombinant human VAMP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-99 aa of BC005941

Sequence: MSTGPTAATGSNRRLQQTQNQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNCEMWAIGITVLVIFIIIIIVWVVS

相同性解析による交差性が予測される生物種
Full Name vesicle-associated membrane protein 3 (cellubrevin)
Calculated molecular weight 11 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC005941
Gene Symbol VAMP3
Gene ID (NCBI) 9341
RRIDAB_2881853
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ15836
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VAMP3, also named cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.