Validation Data Gallery
Tested Applications
| Positive WB detected in | Mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| ELISA | See 3 publications below |
Product Information
30001-0-AP targets VAMP2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein 相同性解析による交差性が予測される生物種 |
| Full Name | vesicle-associated membrane protein 2 (synaptobrevin 2) |
| Calculated molecular weight | 13 kDa |
| GenBank accession number | NM_014232 |
| Gene Symbol | VAMP2 |
| Gene ID (NCBI) | 6844 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P63027 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family of proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2 consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biochem TCEP treatment reduces proteolytic activity of BoNT/B in human neuronal SHSY-5Y cells. | ||
J Cell Biochem The biological activity of ubiquitinated BoNT/B light chain in vitro and in human SHSY-5Y neuronal cells. | ||
Mol Neurobiol L116 Deletion in CSPα Promotes α-Synuclein Aggregation and Neurodegeneration |