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Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using VAC14 Polyclonal antibody (15771-1-AP)

    WB analysis of human spleen using 15771-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 15771-1-AP (VAC14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using VAC14 Polyclonal antibody (15771-1-AP)

    WB analysis of Jurkat using 15771-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15771-1-AP (VAC14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using VAC14 Polyclonal antibody (15771-1-AP)

    IP experiment of Jurkat using 15771-1-AP

    IP result of anti-VAC14 (IP:15771-1-AP, 4ug; Detection:15771-1-AP 1:500) with Jurkat cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human skin tissue using VAC14 Polyclonal antibody (15771-1-AP)

    IHC staining of human skin using 15771-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using VAC14 Polyclonal antibody (15771-1-AP)

    IHC staining of human skin using 15771-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using VAC14 Polyclonal antibody (15771-1-AP)

    IHC staining of human placenta using 15771-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using VAC14 Polyclonal antibody (15771-1-AP)

    IHC staining of human placenta using 15771-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using VAC14 Polyclonal antibody (15771-1-AP)

    IHC staining of human testis using 15771-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using VAC14 Polyclonal antibody (15771-1-AP)

    IHC staining of human testis using 15771-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using VAC14 Polyclonal antibody (15771-1-AP)

    IHC staining of human spleen using 15771-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using VAC14 Polyclonal antibody (15771-1-AP)

    IHC staining of human spleen using 15771-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using VAC14 Polyclonal antibody (15771-1-AP)

    IHC staining of human lung using 15771-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using VAC14 Polyclonal antibody (15771-1-AP)

    IHC staining of human lung using 15771-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using VAC14 Polyclonal antibody (15771-1-AP)

    IHC staining of human ovary using 15771-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using VAC14 Polyclonal antibody (15771-1-AP)

    IHC staining of human ovary using 15771-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 40x lens).

  • Featured Product
  • KD/KO Validated

VAC14 Polyclonal antibody

VAC14 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 15771-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ELISA

Synonyms

ArPIKfyve, Protein VAC14 homolog, Tax1 binding protein 2, TAX1BP2, TRX, VAC14, Vac14 homolog (S. cerevisiae)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman spleen tissue, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman skin tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15771-1-AP targets VAC14 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8415

Product name: Recombinant human VAC14 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 228-576 aa of BC007214

Sequence: KMFRHTDSLFPILLQTLSDESDEVILKDLEVLAEIASSPAGQTDDPGPLDGPDLQASHSELQVPTPGRAGLLNTSGTKGLECSPSTPTMNSYFYKFMINLLKRFSSERKLLEVRGPFIIRQLCLLLNAENIFHSMADILLREEDLKFASTMVHALNTILLTSTELFQLRNQLKDLKTLESQNLFCCLYRSWCHNPVTTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLAEVDKLVQLIECPIFTYLRLQLLDVKNNPYLIKALYGLLMLLPQSSAFQLLSHRLQCVPNPELLQTEDSLKAAPKSQKADSPSIDYAELLQHFEKVQNKHLEVRHQRSGRGDHLDRRVVL

相同性解析による交差性が予測される生物種
Full Name Vac14 homolog (S. cerevisiae)
Calculated molecular weight 88 kDa
Observed molecular weight 88 kDa, 65 kDa
GenBank accession numberBC007214
Gene Symbol VAC14
Gene ID (NCBI) 55697
RRIDAB_2214753
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08AM6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VAC14 antibody 15771-1-APDownload protocol
IHC protocol for VAC14 antibody 15771-1-APDownload protocol
IP protocol for VAC14 antibody 15771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Exp Cell Res

Phosphoinositide phosphatase Sac3 regulates the cell surface expression of scavenger receptor A and formation of lipid droplets in macrophages.

Authors - Shin Morioka
  • KD Validated
View Article
WB

Virology

Ebola virus requires phosphatidylinositol (3,5) bisphosphate production for efficient viral entry.

Authors - Shirley Qiu
  • KO Validated
View Article
humanCoIP

Virulence

Coxiella burnetii effector CvpE maintains biogenesis of Coxiella-containing vacuoles by suppressing lysosome tubulation through binding PI(3)P and perturbing PIKfyve activity on lysosomes

Authors - Mingliang Zhao
View Article