Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:32000 incubated at room temperature for 1.5 hours.

Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

IP result of anti-V5-tag (IP:14440-1-AP, 3ug; Detection:14440-1-AP 1:20000) with Recombinant protein protein lysate 3000ug.