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Validation Data Gallery

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  • Western Blot (WB) analysis of rat lung tissue using Uteroglobin/CC10 Polyclonal antibody (26909-1-AP)

    WB analysis of rat lung using 26909-1-AP (same clone as 26909-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 26909-1-AP (Uteroglobin Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using Uteroglobin/CC10 Polyclonal antibody (26909-1-AP)

    IHC staining of human lung using 26909-1-AP (same clone as 26909-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26909-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using Uteroglobin/CC10 Polyclonal antibody (26909-1-AP)

    IHC staining of human lung using 26909-1-AP (same clone as 26909-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26909-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Uteroglobin/CC10 Polyclonal antibody (26909-1-AP)

    IHC staining of mouse lung using 26909-1-AP (same clone as 26909-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26909-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Uteroglobin/CC10 Polyclonal antibody (26909-1-AP)

    IHC staining of mouse lung using 26909-1-AP (same clone as 26909-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26909-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Uteroglobin/CC10 Polyclonal antibody (26909-1-AP)

    IF Staining of A549 using 26909-1-AP (same clone as 26909-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 26909-1-AP (Uteroglobin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using Uteroglobin/CC10 Polyclonal antibody (26909-1-AP)

    FC experiment of A549 using 26909-1-AP (same clone as 26909-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human Uteroglobin/CC10 (26909-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

Uteroglobin/CC10 Polyclonal antibody, PBS Only

Uteroglobin/CC10 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 26909-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26909-1-PBS targets Uteroglobin/CC10 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25530

Product name: Recombinant human Uteroglobin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 22-91 aa of BC004481

Sequence: EICPSFQRVIETLLMDTPSSYEAAMELFSPDQDMREAGAQLKKLVDTLPQKPRESIIKLMEKIAQSSLCN

相同性解析による交差性が予測される生物種
Full Name secretoglobin, family 1A, member 1 (uteroglobin)
Calculated molecular weight 10 kDa
Observed molecular weight 9 kDa
GenBank accession numberBC004481
Gene Symbol Uteroglobin
Gene ID (NCBI) 7356
RRIDAB_2880680
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP11684
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.

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