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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UTY Recombinant antibody (84332-5-RR)

    WB analysis using 84332-5-RR

    THP-1 cells were subjected to SDS PAGE followed by western blot with 84332-5-RR (UTY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MDA-MB-231 cells using UTY Recombinant antibody (84332-5-RR)

    IF Staining of MDA-MB-231 using 84332-5-RR

    Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using UTY antibody (84332-5-RR, Clone: 241626F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84332-5-RR

    Affinity and Kinetic Characterization of 84332-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84332-5-RR against Human UTY were performed. The affinity constant is 0.23 nM.

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UTY Recombinant antibody

UTY Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84332-5-RR
Clone No.241626F7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

UTY1, KDM6C, KDM6AL, Histone demethylase UTY, 241626F7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells
Positive IF/ICC detected inMDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84332-5-RR targets UTY in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UTY fusion protein Ag35806 相同性解析による交差性が予測される生物種
Full Name ubiquitously transcribed tetratricopeptide repeat gene, Y-linked
Calculated molecular weight150kDa，1347aa
Observed molecular weight130-160kDa
GenBank accession numberNM_007125.4
Gene Symbol UTY
Gene ID (NCBI) 7404
RRIDAB_3671873
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO14607
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UTY (Ubiquitously Transcribed Tetratricopeptide Repeat Containing, Y-Linked) is a protein-coding gene located on the Y chromosome. It is the Y-linked homolog of the X-linked gene KDM6A, which encodes a histone demethylase. UTY is involved in the regulation of gene expression and chromatin structure, similar to its X-linked counterpart KDM6A (PMID: 31097364). UTY is expressed in various tissues and is predicted to be involved in transcription, translation, and nucleic acid binding, which are central to the regulation of gene expression during development, immune function, cell proliferation, and differentiation.

Protocols

Product Specific Protocols
WB protocol for UTY antibody 84332-5-RRDownload protocol
IF protocol for UTY antibody 84332-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols