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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UTP18 Polyclonal antibody (31055-1-AP)

    WB analysis using 31055-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31055-1-AP (UTP18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using UTP18 Polyclonal antibody (31055-1-AP)

    WB analysis using 31055-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31055-1-AP (UTP18 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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UTP18 Polyclonal antibody

UTP18 Polyclonal Antibody for WB, ELISA
Cat No. 31055-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, ELISA

Synonyms

CGI 48, UTP18, WDR50

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, mouse kidney tissue, Jurkat cells, SW480 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31055-1-AP targets UTP18 in WB, ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UTP18 fusion protein Ag34662 相同性解析による交差性が予測される生物種
Full Name UTP18, small subunit (SSU) processome component, homolog (yeast)
Calculated molecular weight62 kDa
Observed molecular weight62 kDa
GenBank accession numberNM_016001
Gene Symbol UTP18
Gene ID (NCBI) 51096
RRIDAB_3669835
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5J1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UTP18 (UTP18 small subunit processome component), also known as WDR50. It is expected to be located in nucleus. The protein is one part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in nucleolar processing of pre-18S ribosomal RNA. The calculated molecular weight of UTP18 is 62 kDa.

Protocols

Product Specific Protocols
WB protocol for UTP18 antibody 31055-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols