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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP54 Polyclonal antibody (26707-1-AP)

    WB analysis using 26707-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26707-1-AP (USP54 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using USP54 Polyclonal antibody (26707-1-AP)

    IHC staining of mouse liver using 26707-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26707-1-AP (USP54 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

USP54 Polyclonal antibody

USP54 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26707-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

C10orf29, Inactive ubiquitin carboxyl-terminal hydrolase 54, Inactive ubiquitin-specific peptidase 54

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26707-1-AP targets USP54 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP54 fusion protein Ag25080 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 54
Observed molecular weight180 kDa
GenBank accession numberBC130633
Gene Symbol USP54
Gene ID (NCBI) 159195
RRIDAB_3669564
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ70EL1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP54 is a ubiquitin-specific protease in the deubiquitinating enzyme (DUB) family that promotes the malignant progression of many cancers. As a deubiquitinating enzyme, USP54 maintains the stability of PLK4 protein through deubiquitination, which promotes centrosome amplification and GC progression. USP54 is also highly expressed in colorectal cancers, and is associated with proliferation, invasion and migration of colorectal cancer. In addition, USP54 plays a key role in the transformation of hormone-sensitive prostate cancer (HSPC) into chemoresistant prostate cancer (CRPC), and may serve as a potential therapeutic target in CRPC.

Protocols

Product Specific Protocols
WB protocol for USP54 antibody 26707-1-APDownload protocol
IHC protocol for USP54 antibody 26707-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols