Validation Data Gallery

Filter: AllWBIP

WB analysis of mouse lung using 15158-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 15158-1-AP
WB result of USP5 antibody (15158-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells.
WB analysis of rat brain using 15158-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 15158-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 15158-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15158-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15158-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15158-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15158-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15158-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 15158-1-AP
IP result of anti-USP5 (IP:15158-1-AP, 4ug; Detection:15158-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

Featured Product
KD/KO Validated

USP5 Polyclonal antibody

USP5 Polyclonal Antibody for WB, IP, ELISA

Cat No. 15158-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, ELISA

Synonyms

Deubiquitinating enzyme 5, EC:3.4.19.12, Isopeptidase T, ISOT, ISOT 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse lung tissue, A549 cells, HeLa cells, human brain tissue, rat lung tissue, rat brain tissue, MDA-MB-453s cells
Positive IP detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

15158-1-AP targets USP5 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7252 Product name: Recombinant human USP5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC005139 Sequence: MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDF 相同性解析による交差性が予測される生物種
Full Name	ubiquitin specific peptidase 5 (isopeptidase T)
Calculated molecular weight	96 kDa
Observed molecular weight	95-105 kDa
GenBank accession number	BC005139
Gene Symbol	USP5
Gene ID (NCBI)	8078
RRID	AB_2213856
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P45974
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.

Protocols

Product Specific Protocols
IP protocol for USP5 antibody 15158-1-AP	Download protocol
WB protocol for USP5 antibody 15158-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IP,IF	Neuron The Deubiquitinating Enzyme USP5 Modulates Neuropathic and Inflammatory Pain by Enhancing Cav3.2 Channel Activity. Authors - Agustin García-Caballero View Article
human	WB	J Exp Med A pathological missense mutation in the deubiquitinase USP5 leads to insensitivity to pain Authors - Flavia T T Antunes View Article
	WB	FASEB J PPP2R1B is modulated by ubiquitination and is essential for spermatogenesis. Authors - Mufeng Du View Article
mouse	WB	Toxicology Critical role of Cav3.2 T-type calcium channels in the peripheral neuropathy induced by bortezomib, a proteasome-inhibiting chemotherapeutic agent, in mice. Authors - Shiori Tomita View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse lung using 15158-1-AP

WB analysis of A549 using 15158-1-AP

WB analysis of rat brain using 15158-1-AP

WB analysis of rat lung using 15158-1-AP

WB analysis of MDA-MB-453s using 15158-1-AP

WB analysis of human brain using 15158-1-AP

WB analysis of human brain using 15158-1-AP

WB analysis of HeLa using 15158-1-AP

WB analysis of HeLa using 15158-1-AP

WB analysis of HeLa using 15158-1-AP

IP experiment of mouse brain using 15158-1-AP

USP5 Polyclonal antibody

USP5 Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The Deubiquitinating Enzyme USP5 Modulates Neuropathic and Inflammatory Pain by Enhancing Cav3.2 Channel Activity.

A pathological missense mutation in the deubiquitinase USP5 leads to insensitivity to pain

PPP2R1B is modulated by ubiquitination and is essential for spermatogenesis.

Critical role of Cav3.2 T-type calcium channels in the peripheral neuropathy induced by bortezomib, a proteasome-inhibiting chemotherapeutic agent, in mice.