Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of A549 using 10473-1-AP
WB result of USP5 antibody (10473-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells.
WB analysis using 10473-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10473-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of U2OS using 10473-1-AP
U2OS cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 10473-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10473-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 10473-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 10473-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of DU 145 using 10473-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10473-1-AP
IP result of anti-USP5 (IP:10473-1-AP, 4ug; Detection:10473-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human heart using 10473-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 10473-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human spleen using 10473-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human spleen using 10473-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 40x lens).

Featured Product
KD/KO Validated

USP5 Polyclonal antibody

USP5 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10473-1-AP

Publications(49)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

Deubiquitinating enzyme 5, EC:3.4.19.12, Isopeptidase T, ISOT, ISOT 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (49)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, mouse brain tissue, human heart tissue, human colon tissue, U2OS cells, DU 145 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human heart tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 18 publications below
WB	See 42 publications below
IHC	See 8 publications below
IF	See 5 publications below
IP	See 6 publications below
ELISA	See 2 publications below
CoIP	See 3 publications below

Product Information

10473-1-AP targets USP5 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0770 Product name: Recombinant human USP5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC004889 Sequence: MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDM 相同性解析による交差性が予測される生物種
Full Name	ubiquitin specific peptidase 5 (isopeptidase T)
Calculated molecular weight	96 kDa
Observed molecular weight	95-105 kDa
GenBank accession number	BC004889
Gene Symbol	USP5
Gene ID (NCBI)	8078
RRID	AB_2272754
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P45974
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 has 2 isoforms with the molecular mass of 96 kDa and 93 kDa.

Protocols

Product Specific Protocols
IHC protocol for USP5 antibody 10473-1-AP	Download protocol
IP protocol for USP5 antibody 10473-1-AP	Download protocol
WB protocol for USP5 antibody 10473-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Commun STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity Authors - Hiroaki Ohishi View Article
human	WB	Exp Mol Med MSK1 promotes colorectal cancer metastasis by increasing Snail protein stability through USP5-mediated Snail deubiquitination Authors - Keun-Seok Hong KD Validated View Article
		Blood The ubiquitin ligase HERC4 mediates c-Maf ubiquitination and delays the growth of multiple myeloma xenografts in nude mice. Authors - Zubin Zhang View Article
human	WB	Theranostics Ubiquitin specific peptidase 5 regulates colorectal cancer cell growth by stabilizing Tu translation elongation factor. Authors - Xin Xu KD Validated View Article
mouse,human	WB	Cell Mol Life Sci P62 promotes FSH-induced antral follicle formation by directing degradation of ubiquitinated WT1 Authors - Ting Zhao KD Validated View Article
human	WB	Clin Transl Med MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma. Authors - Mengqing Xiao KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 10473-1-AP

WB analysis using 10473-1-AP

WB analysis of A549 using 10473-1-AP

WB analysis of U2OS using 10473-1-AP

WB analysis of MDA-MB-453s using 10473-1-AP

WB analysis of mouse brain using 10473-1-AP

WB analysis of human heart using 10473-1-AP

WB analysis of human colon using 10473-1-AP

WB analysis of DU 145 using 10473-1-AP

IP experiment of mouse brain using 10473-1-AP

IHC staining of human heart using 10473-1-AP

IHC staining of human heart using 10473-1-AP

IHC staining of human spleen using 10473-1-AP

IHC staining of human spleen using 10473-1-AP

USP5 Polyclonal antibody

USP5 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity

MSK1 promotes colorectal cancer metastasis by increasing Snail protein stability through USP5-mediated Snail deubiquitination

The ubiquitin ligase HERC4 mediates c-Maf ubiquitination and delays the growth of multiple myeloma xenografts in nude mice.

Ubiquitin specific peptidase 5 regulates colorectal cancer cell growth by stabilizing Tu translation elongation factor.

P62 promotes FSH-induced antral follicle formation by directing degradation of ubiquitinated WT1

MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma.