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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP4 Monoclonal antibody (66822-1-Ig)

    WB analysis using 66822-1-Ig (same clone as 66822-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66822-1-Ig (USP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using USP4 Monoclonal antibody (66822-1-Ig)

    WB analysis using 66822-1-Ig (same clone as 66822-1-PBS)

    HEK-293 cells and Jurkat cells were subjected to SDS PAGE followed by western blot with 66822-1-Ig (USP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using USP4 Monoclonal antibody (66822-1-Ig)

    IHC staining of human breast cancer using 66822-1-Ig (same clone as 66822-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66822-1-Ig (USP4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using USP4 Monoclonal antibody (66822-1-Ig)

    IHC staining of human breast cancer using 66822-1-Ig (same clone as 66822-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66822-1-Ig (USP4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using USP4 Monoclonal antibody (66822-1-Ig)

    IHC staining of mouse kidney using 66822-1-Ig (same clone as 66822-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66822-1-Ig (USP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using USP4 Monoclonal antibody (66822-1-Ig)

    IHC staining of mouse kidney using 66822-1-Ig (same clone as 66822-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66822-1-Ig (USP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using USP4 Monoclonal antibody (66822-1-Ig)

    IF Staining of human breast cancer using 66822-1-Ig (same clone as 66822-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using USP4 antibody (66822-1-Ig, Clone: 1G11G8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using USP4 Monoclonal antibody (66822-1-Ig)

    IF Staining of human liver cancer using 66822-1-Ig (same clone as 66822-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using USP4 antibody (66822-1-Ig, Clone: 1G11G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of THP-1 cells using USP4 Monoclonal antibody (66822-1-Ig)

    FC experiment of THP-1 using 66822-1-Ig (same clone as 66822-1-PBS)

    1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human USP4 (66822-1-Ig, Clone:1G11G8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

USP4 Monoclonal antibody, PBS Only

USP4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 66822-1-PBS
Clone No.1G11G8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Synonyms

1G11G8, EC:3.4.19.12, Ubiquitin carboxyl-terminal hydrolase 4, Ubiquitin-specific-processing protease 4, UNP

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66822-1-PBS targets USP4 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28297

Product name: Recombinant human USP4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 567-779 aa of BC125131

Sequence: VCSTSVDGSECVTLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVALR

相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 4 (proto-oncogene)
Calculated molecular weight 963 aa, 109 kDa
Observed molecular weight 109 kDa
GenBank accession numberBC125131
Gene Symbol USP4
Gene ID (NCBI) 7375
RRIDAB_2882165
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13107
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

USP4, also named as UNP and UNPH, belongs to the peptidase C19 family and USP4 subfamily. USP4 is a Deubiquitinating enzymes that links to mitogen-activated protein kinase signaling, pre-mRNA splicing and control of p53 stability (PMID: 26455393). USP4 has 3 isoforms with the molecular mass of 36, 104 and 109 kDa. Recently, it has been reported that USP4 is a critical factor in promoting lung cancer stemness and potentially useful lung cancer prognosis marker (PMID: 32549341).

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