Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse spleen tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 13 publications below |
| IHC | See 5 publications below |
| IF | See 4 publications below |
| IP | See 2 publications below |
Product Information
18465-1-AP targets USP37 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12513 Product name: Recombinant human USP37 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC112901 Sequence: MLTLQDNSFLSIDKVPSKDAEEMRLFLDAVHQNRLPAAMKPSQGSGSFGAILGSRTSQKETSRQLSYSDNQASAKRGSLETKDDIPFRKVLGNPGRGSIKTVAGSGIARTIPSLTSTSTPLRSGLLENRTEKRKRMISTGSELNEDYPKENDSSSNNKAMTDPSRKYLTSSREKQLSLKQSEENRTSGLLPLQSSSFYGSRAGSKEHSSGGTNLDRTNVSSQTPSAKRSLGFLPQPVPLSVKKLRCNQDYTGWNKPRVPLSSHQQQQLQGFSNLGNTCYMNAILQSLFSLQSFANDLLKQGIPWKKIPLNALIRRFAHLLVKKDICNSETKKDLLKKVKNAISATAERFSG 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin specific peptidase 37 |
| Calculated molecular weight | 885 aa, 100 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC112901 |
| Gene Symbol | USP37 |
| Gene ID (NCBI) | 57695 |
| RRID | AB_10598483 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86T82 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USP37 (ubiquitin specific peptidase 37), is a member of ubiquitin-specific processing proteases family. Human USP37, localized mainly in the cytoplasm, is composed of 979 amino acids harboring three ubiquitin-interacting motifs between the Cys box and His box of the primary sequence (PMID: 27045719, 24324262). High levels of USP37 gene expression in lung cancer promoted cell viability as well as the Warburg effect via deubiquitination and stabilization of pluripotent factor c-Myc protein (PMID: 25284584). Moreover, USP37 expression was upregulated in breast cancer tissues compared with surrounding tissues and its overexpression was significantly correlated with increased rates of mortality. USP37 may represent a novel molecular target for breast cancer treatment (PMID: 30482232, 27045719).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for USP37 antibody 18465-1-AP | Download protocol |
| IP protocol for USP37 antibody 18465-1-AP | Download protocol |
| WB protocol for USP37 antibody 18465-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res USP37 regulates DNA damage response through stabilizing and deubiquitinating BLM.
| ||
Proc Natl Acad Sci U S A CYLD regulates spindle orientation by stabilizing astral microtubules and promoting dishevelled-NuMA-dynein/dynactin complex formation. | ||
Cell Death Dis USP3 promotes DNA damage response and chemotherapy resistance through stabilizing and deubiquitinating SMARCA5 in prostate cancer | ||
Theranostics PLAGL2 promotes the proliferation and migration of gastric cancer cells via USP37-mediated deubiquitination of Snail1.
| ||
Front Immunol Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses.
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