Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, RAW 264.7 cells |
| Positive IHC detected in | human colon cancer tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
24559-1-AP targets USP35 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20002 Product name: Recombinant human USP35 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-342 aa of BC131489 Sequence: MPGLRRRASWRVSIPGSWPSQTRARLVSSTWATHAMSTASFRPYSWRLSSFRHCVLRLTENNSQPLMTKLQWLFGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHPDAAIPSGERTCGSE 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin specific peptidase 35 |
| Calculated molecular weight | 1018 aa, 113 kDa |
| Observed molecular weight | 84 kDa |
| GenBank accession number | BC131489 |
| Gene Symbol | USP35 |
| Gene ID (NCBI) | 57558 |
| RRID | AB_2879608 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P2H5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USP35 is a member of the peptidase C19 family. USP35 is involved in cell cycle regulation, cell fate, and cancer development. USP35 regulates cell mitosis by deubiquitinating Aurora B and prevents its degradation from APC/CDH. USP35 can also regulate MFN2 abundance and PARK2-mediated mitophagy. USP35 has 2 isoforms with the molecular mass of 84 and 113 kDa (PMID: 34618999).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for USP35 antibody 24559-1-AP | Download protocol |
| WB protocol for USP35 antibody 24559-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Commun Biol Overexpression of USP35 enhances the protective effect of hUC-MSCs and their extracellular vesicles in oxygen-glucose deprivation/reperfusion-induced SH-SY5Y cells via stabilizing FUNDC1
| ||
Am J Physiol Cell Physiol USP35 promotes breast cancer progression by regulating PFK-1 ubiquitination to mediate glycolysis | ||
Transl Oncol USP35 promotes hepatocellular carcinoma proliferation through GASC1-mediated ROCK2 upregulation
| ||
Cell Death Discov Deubiquitinase USP35 stabilizes BRPF1 to activate mevalonate (MVA) metabolism during prostate tumorigenesis | ||
Oncogenesis USP35 promotes cell proliferation and chemotherapeutic resistance through stabilizing FUCA1 in colorectal cancer |
