Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IHC detected in | mouse testis tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
18838-1-AP targets USP32 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin specific peptidase 32 |
| Calculated molecular weight | 182 kDa |
| Observed molecular weight | 230 kDa |
| GenBank accession number | BC094680 |
| Gene Symbol | USP32 |
| Gene ID (NCBI) | 84669 |
| RRID | AB_10598326 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NFA0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USP32 belongs to the peptidase C19 family. USP32 catalytic the reaction :Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for USP32 antibody 18838-1-AP | Download protocol |
| WB protocol for USP32 antibody 18838-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Mol Basis Dis USP46 enhances tamoxifen resistance in breast cancer cells by stabilizing PTBP1 to facilitate glycolysis | ||
Acta Biochim Biophys Sin (Shanghai) Roles of the hsa_circ_0013880/USP32/Rap1b axis in the proliferation and apoptosis of acute myeloid leukemia cells
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