Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 12490-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12490-1-AP (USP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12490-1-AP
WB result of USP3 antibody (12490-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP3 transfected K-562 cells.
WB analysis of K-562 using 12490-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12490-1-AP (USP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 12490-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 12490-1-AP (USP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 12490-1-AP
IP result of anti-USP3 (IP:12490-1-AP, 4ug; Detection:12490-1-AP 1:500) with mouse liver tissue lysate 4000ug.
IHC staining of human stomach cancer using 12490-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12490-1-AP (USP3 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
IF Staining of HeLa using 12490-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12490-1-AP (USP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 12490-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12490-1-AP (USP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 12490-1-AP
Immunofluorescent analysis of MCF-7 cells using 12490-1-AP (USP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

USP3 Polyclonal antibody

USP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12490-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

EC:3.4.19.12, SIH003, Ubiquitin carboxyl-terminal hydrolase 3, Ubiquitin thioesterase 3, Ubiquitin-specific-processing protease 3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, K-562 cells, RAW264.7, HepG2 cells, Jurkat cells
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 13 publications below
IHC	See 3 publications below
IF	See 5 publications below
IP	See 2 publications below
CoIP	See 3 publications below

Product Information

12490-1-AP targets USP3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3085 Product name: Recombinant human USP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC018113 Sequence: MECPHLSSSVCIAPDSAKFPNGSPSSWCCSVCRSNKSPWVCLTCSSVHCGRYVNGHAKKHYEDAQVPLTNHKKSEKQDKVQHTVCMDCSSYSTYCYRCDDFVVNDTKLGLVQKVREHLQNLENSAFTADRHKKRKLLENSTLNSKLLKVNGSTTAICATGLRNLGNTCFMNAILQSLSNIEQFCCYFKELPAVELRNGKTAGRRTYHTRSQGDNNVSLVEEFRKTLCALWQGSQTAFSPESLFYVVWKIMPNFRGYQQQDAHEFMRYLLDHLHLELQGGFNGVSRSAILQENSTLSASNK 相同性解析による交差性が予測される生物種
Full Name	ubiquitin specific peptidase 3
Calculated molecular weight	520 aa, 59 kDa
Observed molecular weight	53-59 kDa
GenBank accession number	BC018113
Gene Symbol	USP3
Gene ID (NCBI)	9960
RRID	AB_10639042
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y6I4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

USP3(Ubiquitin carboxyl-terminal hydrolase 3) belongs to the peptidase C19 family. It may regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. USP3 is implicated as a novel chromatin modifier in the maintenance of genome integrity. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for USP3 antibody 12490-1-AP	Download protocol
IHC protocol for USP3 antibody 12490-1-AP	Download protocol
IF protocol for USP3 antibody 12490-1-AP	Download protocol
IP protocol for USP3 antibody 12490-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Cell Death Differ N6-methyladenosine-modified USP13 induces pro-survival autophagy and imatinib resistance via regulating the stabilization of autophagy-related protein 5 in gastrointestinal stromal tumors Authors - Zhishuang Gao View Article
human	WB	EMBO J A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression. Authors - Doowon Huh View Article
human	WB	BMC Med Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis Authors - Zhijian Zheng View Article
human	WB,IHC,IF	Cell Death Dis USP3 promotes DNA damage response and chemotherapy resistance through stabilizing and deubiquitinating SMARCA5 in prostate cancer Authors - Sheng Li KD Validated View Article
rat	WB	Cell Rep Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex Authors - Kellen D Winden View Article
human	WB	BMC Cancer Protein deubiquitylase USP3 stabilizes Aurora A to promote proliferation and metastasis of esophageal squamous cell carcinoma. Authors - Ke Shi KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12490-1-AP

WB analysis of K-562 using 12490-1-AP

WB analysis of K-562 using 12490-1-AP

WB analysis of RAW264.7 using 12490-1-AP

IP experiment of mouse liver using 12490-1-AP

IHC staining of human stomach cancer using 12490-1-AP

IF Staining of HeLa using 12490-1-AP

IF Staining of HepG2 using 12490-1-AP

IF Staining of MCF-7 using 12490-1-AP

USP3 Polyclonal antibody

USP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

N6-methyladenosine-modified USP13 induces pro-survival autophagy and imatinib resistance via regulating the stabilization of autophagy-related protein 5 in gastrointestinal stromal tumors

A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression.

Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis

USP3 promotes DNA damage response and chemotherapy resistance through stabilizing and deubiquitinating SMARCA5 in prostate cancer

Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex

Protein deubiquitylase USP3 stabilizes Aurora A to promote proliferation and metastasis of esophageal squamous cell carcinoma.