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  • Featured Product
  • KD/KO Validated

USP29 Polyclonal antibody

USP29 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27522-1-AP

Synonyms

Deubiquitinating enzyme 29, HOM TES 84/86, Ubiquitin thiolesterase 29, USP29

Validation Data Gallery

Applications:
  • WB analysis using 27522-1-AP

    WB analysis using 27522-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27522-1-AP (USP29 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 27522-1-AP

    WB analysis of mouse testis using 27522-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 27522-1-AP (USP29 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 27522-1-AP

    IHC staining of mouse testis using 27522-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27522-1-AP (USP29 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 27522-1-AP

    IHC staining of mouse testis using 27522-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27522-1-AP (USP29 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27522-1-AP targets USP29 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP29 fusion protein Ag23231 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 29
Observed molecular weight 104 kDa
GenBank accession numberBC130394
Gene symbol USP29
Gene ID (NCBI) 57663
RRIDAB_2880898
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for USP29 antibody 27522-1-APDownload protocol
IHC protocol for USP29 antibody 27522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Ubiquitin-Specific Protease 29 Exacerbates Cerebral Ischemia-Reperfusion Injury in Mice.

Authors - Jia-Bao Hou
  • KO Validated
View Article