Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HepG2 using 13126-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13126-1-AP (USP24 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 13126-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 13126-1-AP (USP24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 13126-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 13126-1-AP (USP24 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 13126-1-AP
IP result of anti-USP24 (IP:13126-1-AP, 4ug; Detection:13126-1-AP 1:3000) with mouse brain tissue lysate 1680 ug.
IHC staining of human lymphoma using 13126-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13126-1-AP (USP24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 13126-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13126-1-AP (USP24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 13126-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13126-1-AP (USP24 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 13126-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13126-1-AP (USP24 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 13126-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13126-1-AP (USP24 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 13126-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13126-1-AP (USP24 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat pancreas using 13126-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 13126-1-AP (USP24 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 13126-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13126-1-AP (USP24 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 13126-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13126-1-AP (USP24 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 13126-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13126-1-AP (USP24 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 13126-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using USP24 antibody (13126-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of HeLa using 13126-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP24 antibody (13126-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

USP24 Polyclonal antibody

USP24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13126-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Deubiquitinating enzyme 24, EC:3.4.19.12, KIAA1057, Ubiquitin carboxyl-terminal hydrolase 24, Ubiquitin thioesterase 24

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (25)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, mouse lung tissue, rat lung tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human lymphoma tissue, rat stomach tissue, human placenta tissue, rat pancreas tissue, mouse liver tissue, mouse stomach tissue, mouse pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 20 publications below
IHC	See 6 publications below
IF	See 4 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

13126-1-AP targets USP24 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3779 Product name: Recombinant human USP24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2312-2620 aa of BC029660 Sequence: MISFLLGASRQNNQIRRWSSAQAREFGNLHNTVALLVLHSDVSSQRNVAPGIFKQRPPISIAPSSPLLPLHEEVEALLFMSEGKPYLLEVMFALRELTGSLLALIEMVVYCCFCNEHFSFTMLHFIKNQLETAPPHELKNTFQLLHEILVIEDPIQVERVKFVFETENGLLALMHHSNHVDSSRCYQCVKFLVTLAQKCPAAKEYFKENSHHWSWAVQWLQKKMSEHYWTPQSNVSNETSTGKTFQRTISAQDTLAYATALLNEKEQSGSSNGSESSPANENGDRHLQQGSESPMMIGELRSDLDDVDP 相同性解析による交差性が予測される生物種
Full Name	ubiquitin specific peptidase 24
Calculated molecular weight	294 kDa
Observed molecular weight	280-294 kDa
GenBank accession number	BC029660
Gene Symbol	USP24
Gene ID (NCBI)	23358
RRID	AB_2212761
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UPU5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for USP24 antibody 13126-1-AP	Download protocol
IHC protocol for USP24 antibody 13126-1-AP	Download protocol
IP protocol for USP24 antibody 13126-1-AP	Download protocol
WB protocol for USP24 antibody 13126-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Signal Transduct Target Ther Aberrant Notch-signaling promotes tumor angiogenesis in esophageal squamous-cell carcinoma Authors - Cainan Li View Article
human	WB	Mol Cancer LINE-1 promotes tumorigenicity and exacerbates tumor progression via stimulating metabolism reprogramming in non-small cell lung cancer Authors - Zeguo Sun View Article
human	WB,IF	Autophagy The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability. Authors - Julia A Thayer KD Validated View Article
human	WB,IHC,IF	Nat Commun USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy. Authors - Yi-Chang Wang KD Validated View Article
mouse		Nat Commun ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP. Authors - Francesca Bufalieri View Article
mouse	WB	Nat Commun A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation. Authors - Jung-Min Kim KD Validated View Article

Validation Data Gallery

WB analysis of HepG2 using 13126-1-AP

WB analysis of rat lung using 13126-1-AP

WB analysis of mouse lung using 13126-1-AP

IP experiment of mouse brain using 13126-1-AP

IHC staining of human lymphoma using 13126-1-AP

IHC staining of human lymphoma using 13126-1-AP

IHC staining of mouse liver using 13126-1-AP

IHC staining of human placenta using 13126-1-AP

IHC staining of mouse pancreas using 13126-1-AP

IHC staining of mouse stomach using 13126-1-AP

IHC staining of rat pancreas using 13126-1-AP

IHC staining of rat stomach using 13126-1-AP

IHC staining of mouse stomach using 13126-1-AP

IHC staining of rat stomach using 13126-1-AP

IF Staining of A431 using 13126-1-AP

IF Staining of HeLa using 13126-1-AP

USP24 Polyclonal antibody

USP24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Aberrant Notch-signaling promotes tumor angiogenesis in esophageal squamous-cell carcinoma

LINE-1 promotes tumorigenicity and exacerbates tumor progression via stimulating metabolism reprogramming in non-small cell lung cancer

The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability.

USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy.

ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP.

A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.

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