Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human lung tissue, human placenta tissue, rat stomach tissue, human spleen tissue, rat cerebellum tissue, human brain tissue, human testis tissue, human stomach cancer tissue, human ovary tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 12 publications below |
| WB | See 22 publications below |
| IHC | See 7 publications below |
| IF | See 3 publications below |
| IP | See 5 publications below |
Product Information
17491-1-AP targets USP20 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11590 Product name: Recombinant human USP20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC039593 Sequence: MGDSRDLCPHLDSIGEVTKEDLLLKSKGTCQSCGVTGPNLWACLQVACPYVGCGESFADHSTIHAQAKKHNLTVNLTTFRLWCYACEKEVFLEQRLAAPLLGSSSKFSEQDSPPPSHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLVSEVWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPVVATVALTEARDSDSSDTDEKREGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSQAETELLIPDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVD 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin specific peptidase 20 |
| Calculated molecular weight | 914 aa, 102 kDa |
| Observed molecular weight | 110-120 kDa |
| GenBank accession number | BC039593 |
| Gene Symbol | USP20 |
| Gene ID (NCBI) | 10868 |
| RRID | AB_2212570 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2K6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USP20(ubiquitin carboxyl-terminal hydrolase 20) is also named as KIAA1003, LSFR3A, VDU2 and belongs to the peptidase C19 family and the USP20/USP33 subfamily. It can specifically deubiquitinate and stabilize HIF1A and, therefore, increase expression of HIF-1alpha targeted genes, such as vascular endothelial growth factor (VEGF). It also plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for USP20 antibody 17491-1-AP | Download protocol |
| IP protocol for USP20 antibody 17491-1-AP | Download protocol |
| WB protocol for USP20 antibody 17491-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Targeting histone deacetylase suppresses tumor growth through eliciting METTL14-modified m6 A RNA methylation in ocular melanoma | ||
Acta Pharm Sin B USP20 as a super-enhancer-regulated gene drives T-ALL progression via HIF1A deubiquitination.
| ||
MedComm (2020) ATR-dependent ubiquitin-specific protease 20 phosphorylation confers oxaliplatin and ferroptosis resistance
| ||
Cell Rep Med Targeting USP21 to inhibit abdominal aortic aneurysm progression by suppressing the phenotypic transition of vascular smooth muscle cells | ||
Cell Death Differ USP20 positively regulates tumorigenesis and chemoresistance through β-catenin stabilization.
| ||
Cell Death Discov Autophagy dysregulation via the USP20-ULK1 axis in the HERC2-related neurodevelopmental disorder |