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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using USP19 Polyclonal antibody (25768-1-AP)

    WB analysis of Jurkat using 25768-1-AP (same clone as 25768-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using USP19 Polyclonal antibody (25768-1-AP)

    WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using USP19 Polyclonal antibody (25768-1-AP)

    WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USP19 Polyclonal antibody (25768-1-AP)

    WB analysis of HeLa using 25768-1-AP (same clone as 25768-1-PBS)

    WB result of USP19 antibody (25768-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP19 transfected HeLa cells. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using USP19 Polyclonal antibody (25768-1-AP)

    WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using USP19 Polyclonal antibody (25768-1-AP)

    IP experiment of Jurkat using 25768-1-AP (same clone as 25768-1-PBS)

    IP result of anti-USP19 (IP:25768-1-AP, 4ug; Detection:25768-1-AP 1:500) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

USP19 Polyclonal antibody, PBS Only

USP19 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 25768-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

EC:3.4.19.12, KIAA0891, Ubiquitin carboxyl-terminal hydrolase 19, Ubiquitin thioesterase 19, Ubiquitin-specific-processing protease 19

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25768-1-PBS targets USP19 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22646

Product name: Recombinant human USP19 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 203-500 aa of BC146752

Sequence: VEADEQLCIPPLNSQTCLLGSEENLAPLAGEKAVPPGNDPVSPAMVRSRNPGKDDCAKEEMAVAADAATLVDEPESMVNLAFVKNDSYEKGPDSVVVHVYVKEICRDTSRVLFREQDFTLIFQTRDGNFLRLHPGCGPHTTFRWQVKLRNLIEPEQCTFCFTASRIDICLRKRQSQRWGGLEAPAARVGGAKVAVPTGPTPLDSTPPGGAPHPLTGQEEARAVEKDKSKARSEDTGLDSVATRTPMEHVTPKPETHLASPKPTCMVPPMPHSPVSGDSVEEEEEEEKKVCLPGFTGLV

相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 19
Calculated molecular weight 1318 aa, 146 kDa
Observed molecular weight 70 kDa, 120 kDa, 150-180 kDa
GenBank accession numberBC146752
Gene Symbol USP19
Gene ID (NCBI) 10869
RRIDAB_2713918
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO94966
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ubiquitin-specific protease (USP)19 is a recently identified deubiquitinating enzyme (DUB) having multiple splice variants and cellular functions. Since the sum of molecular weights of the two small bands (65 kDa+120 kDa) approximates the size of the full-length protein (~185 kDa), USP19 is likely to be endoproteolytically cleaved. Interestingly, the small bands were not observed when the DUB activity was inactivated, suggesting that the cleavage of USP19 depends on its DUB activity (PMID: 25088257).

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