Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 16840-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16840-1-AP
WB result of USP13 antibody (16840-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP13 transfected HeLa cells.
WB analysis of rat testis using 16840-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 16840-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16840-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 16840-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16840-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16840-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 16840-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16840-1-AP
WB result of USP13 antibody (16840-1-AP, 1:1000) with si-Control and si-USP13 transfected HeLa cells.
IP experiment of HeLa using 16840-1-AP
IP result of anti-USP13 (IP:16840-1-AP, 5ug; Detection:16840-1-AP 1:500) with HeLa cells lysate 2000ug.
IHC staining of human testis using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin cancer using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16840-1-AP (USP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16840-1-AP (USP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin cancer using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16840-1-AP (USP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 16840-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16840-1-AP (USP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 16840-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using USP13 antibody (16840-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

USP13 Polyclonal antibody

USP13 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16840-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

EC:3.4.19.12, Isopeptidase T-3, ISOT3, ISOT-3, Ubiquitin carboxyl-terminal hydrolase 13

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (20)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse testis tissue, A375 cells, HEK-293 cells, rat testis tissue, PC-3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human skin cancer tissue, human brain tissue, human heart tissue, human liver tissue, human ovary tissue, human placenta tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 18 publications below
IHC	See 3 publications below
IF	See 2 publications below
IP	See 2 publications below
CoIP	See 2 publications below

Product Information

16840-1-AP targets USP13 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10527 Product name: Recombinant human USP13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 515-863 aa of BC016146 Sequence: IAYELTRREAEANRRPLPELVRAKIPFSACLQAFSEPENVDDFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQPGEEELPDISPPIVIPDDSKDRLMNQLIDPSDIDESSVMQLAEMGFPLEACRKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFGASGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFEEDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMSGHYICHIKKEGRWVIYNDHKVCASERPPKDLGYMYFYRRIPS 相同性解析による交差性が予測される生物種
Full Name	ubiquitin specific peptidase 13 (isopeptidase T-3)
Calculated molecular weight	863 aa, 97 kDa
Observed molecular weight	97-100 kDa
GenBank accession number	BC016146
Gene Symbol	USP13
Gene ID (NCBI)	8975
RRID	AB_2214569
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92995
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for USP13 antibody 16840-1-AP	Download protocol
IHC protocol for USP13 antibody 16840-1-AP	Download protocol
IP protocol for USP13 antibody 16840-1-AP	Download protocol
WB protocol for USP13 antibody 16840-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Genes Dev Chaperone-mediated autophagy degrades mutant p53. Authors - Vakifahmetoglu-Norberg Helin H View Article
mouse,human	WB,IHC,IF	EBioMedicine The deubiquitinase USP13 stabilizes the anti-inflammatory receptor IL-1R8/Sigirr to suppress lung inflammation. Authors - Lian Li KD Validated View Article
mouse,human	WB,IF	Transl Res Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells. Authors - Xinxin Liao KO Validated View Article
human	WB	J Cell Biol Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death. Authors - Hong-Guang Xia View Article
human	WB,IP	Oncogene USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation Authors - Cunying Ma KD Validated View Article
mouse,human	WB	J Cell Physiol Lipopolysaccharide reduces USP13 stability through c-Jun N-terminal kinase activation in Kupffer cells. Authors - Fan Yu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16840-1-AP

WB analysis of HeLa using 16840-1-AP

WB analysis of rat testis using 16840-1-AP

WB analysis of A375 using 16840-1-AP

WB analysis of HEK-293 using 16840-1-AP

WB analysis of mouse testis using 16840-1-AP

WB analysis of HeLa using 16840-1-AP

WB analysis of HEK-293 using 16840-1-AP

WB analysis of PC-3 using 16840-1-AP

WB analysis of HeLa using 16840-1-AP

IP experiment of HeLa using 16840-1-AP

IHC staining of human testis using 16840-1-AP

IHC staining of human testis using 16840-1-AP

IHC staining of human skin cancer using 16840-1-AP

IHC staining of human skin cancer using 16840-1-AP

IHC staining of human heart using 16840-1-AP

IHC staining of human heart using 16840-1-AP

IHC staining of human placenta using 16840-1-AP

IHC staining of human placenta using 16840-1-AP

IHC staining of human brain using 16840-1-AP

IHC staining of human brain using 16840-1-AP

IHC staining of human liver using 16840-1-AP

IHC staining of human liver using 16840-1-AP

IHC staining of human ovary using 16840-1-AP

IHC staining of human ovary using 16840-1-AP

IHC staining of human skin cancer using 16840-1-AP

IHC staining of human skin cancer using 16840-1-AP

IF Staining of HeLa using 16840-1-AP

USP13 Polyclonal antibody

USP13 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Chaperone-mediated autophagy degrades mutant p53.

The deubiquitinase USP13 stabilizes the anti-inflammatory receptor IL-1R8/Sigirr to suppress lung inflammation.

Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.

Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death.

USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation

Lipopolysaccharide reduces USP13 stability through c-Jun N-terminal kinase activation in Kupffer cells.