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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP11 Polyclonal antibody (22340-1-AP)

    WB analysis using 22340-1-AP (same clone as 22340-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using USP11 Polyclonal antibody (22340-1-AP)

    WB analysis of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)

    WB result of USP11 antibody (22340-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP11 transfected HEK-293 cells. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using USP11 Polyclonal antibody (22340-1-AP)

    WB analysis of A549 using 22340-1-AP (same clone as 22340-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using USP11 Polyclonal antibody (22340-1-AP)

    WB analysis of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using USP11 Polyclonal antibody (22340-1-AP)

    WB analysis of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using USP11 Polyclonal antibody (22340-1-AP)

    WB analysis of human testis using 22340-1-AP (same clone as 22340-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using USP11 Polyclonal antibody (22340-1-AP)

    WB analysis of human testis using 22340-1-AP (same clone as 22340-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using USP11 Polyclonal antibody (22340-1-AP)

    WB analysis of Jurkat using 22340-1-AP (same clone as 22340-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using USP11 Polyclonal antibody (22340-1-AP)

    IP experiment of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)

    IP result of anti-USP11 (IP:22340-1-AP, 4ug; Detection:22340-1-AP 1:2000) with HEK-293 cells lysate 2400ug. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using USP11 Polyclonal antibody (22340-1-AP)

    IHC staining of human breast cancer using 22340-1-AP (same clone as 22340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using USP11 Polyclonal antibody (22340-1-AP)

    IHC staining of human breast cancer using 22340-1-AP (same clone as 22340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using USP11 Polyclonal antibody (22340-1-AP)

    IHC staining of human liver cancer using 22340-1-AP (same clone as 22340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using USP11 Polyclonal antibody (22340-1-AP)

    IHC staining of human liver cancer using 22340-1-AP (same clone as 22340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using USP11 Polyclonal antibody (22340-1-AP)

    IHC staining of human testis using 22340-1-AP (same clone as 22340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using USP11 Polyclonal antibody (22340-1-AP)

    IHC staining of human testis using 22340-1-AP (same clone as 22340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using USP11 Polyclonal antibody (22340-1-AP)

    IHC staining of human brain using 22340-1-AP (same clone as 22340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using USP11 Polyclonal antibody (22340-1-AP)

    IHC staining of human brain using 22340-1-AP (same clone as 22340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using USP11 Polyclonal antibody (22340-1-AP)

    IF Staining of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using USP11 antibody (22340-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

USP11 Polyclonal antibody, PBS Only

USP11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 22340-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Deubiquitinating enzyme 11, EC:3.4.19.12, Ubiquitin carboxyl-terminal hydrolase 11, Ubiquitin thioesterase 11, Ubiquitin thiolesterase 11

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22340-1-PBS targets USP11 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17829

Product name: Recombinant human USP11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 441-743 aa of BC000350

Sequence: YLSVPLLISHKRVLEVFFIPMDPRRKPEQHRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPLSSILDRDDIFVYEVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYYGLMLFGHPLLVSVPRDRFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKEDDEEDKDDVPGPSTGGSLRDPEPEQAGPSSGVTNRCPFLLDNCLGTSQWPPRRRRKQLFTLQTVNSNGTSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEAEGYVKHDCVGYVMKKAPVRLQECI

相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 11
Calculated molecular weight 963 aa, 110 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC000350
Gene Symbol USP11
Gene ID (NCBI) 8237
RRIDAB_2879079
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP51784
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

USP11(Ubiquitin carboxyl-terminal hydrolase 11) is also named as UHX1 and belongs to the peptidase C19 family. It is a deubiquitinating enzyme that controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction.

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