Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 13509-1-AP
WB result of p115, USO1 antibody (13509-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p115, USO1 transfected HeLa cells.
WB analysis using 13509-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 13509-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 13509-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13509-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 13509-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 13509-1-AP
IP result of anti-p115, USO1 (IP:13509-1-AP, 3ug; Detection:13509-1-AP 1:1500) with mouse brain tissue lysate 7000ug.
IHC staining of human gliomas using 13509-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13509-1-AP (p115, USO1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 13509-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13509-1-AP (p115, USO1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 13509-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (13509-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13509-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (13509-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13509-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13509-1-AP (p115, USO1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13509-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13509-1-AP (p115, USO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

p115, USO1 Polyclonal antibody

p115, USO1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13509-1-AP

Publications(26)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

USO1, VDP, Transcytosis-associated protein, TAP, Protein USO1 homolog

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (26)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse testis tissue, mouse thymus tissue, HeLa cells, human brain tissue, SH-SY5Y cells, HepG2 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 17 publications below
IF	See 18 publications below
CoIP	See 1 publications below

Product Information

13509-1-AP targets p115, USO1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4431 Product name: Recombinant human p115, USO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 660-961 aa of BC032654 Sequence: EQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLADQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI 相同性解析による交差性が予測される生物種
Full Name	USO1 homolog, vesicle docking protein (yeast)
Calculated molecular weight	962 aa, 108 kDa
Observed molecular weight	115 kDa
GenBank accession number	BC032654
Gene Symbol	USO1
Gene ID (NCBI)	8615
RRID	AB_2257094
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O60763
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).

Protocols

Product Specific Protocols
IF protocol for p115, USO1 antibody 13509-1-AP	Download protocol
IHC protocol for p115, USO1 antibody 13509-1-AP	Download protocol
IP protocol for p115, USO1 antibody 13509-1-AP	Download protocol
WB protocol for p115, USO1 antibody 13509-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Mol Cell An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress. Authors - Julian Nüchel View Article
human	IF	Nat Commun AmotL2 disrupts apical-basal cell polarity and promotes tumour invasion. Authors - Mahdi Mojallal View Article
human	WB,IF	J Cell Biol Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure. Authors - Yijun Zhang View Article
human	WB	J Cell Biol PMID: 29133483 Authors - Junwan Fan View Article
human	WB	PLoS Pathog Defining the proteolytic landscape during enterovirus infection. Authors - Mohsan Saeed View Article
mouse	IF	Cell Death Dis Globozoospermia and lack of acrosome formation in GM130-deficient mice. Authors - Feng Han View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 13509-1-AP

WB analysis using 13509-1-AP

WB analysis of mouse testis using 13509-1-AP

WB analysis of mouse thymus using 13509-1-AP

WB analysis of human brain using 13509-1-AP

WB analysis of SH-SY5Y using 13509-1-AP

IP experiment of mouse brain using 13509-1-AP

IHC staining of human gliomas using 13509-1-AP

IHC staining of human gliomas using 13509-1-AP

IF Staining of HeLa using 13509-1-AP

IF Staining of HeLa using 13509-1-AP

IF Staining of HeLa using 13509-1-AP

IF Staining of HeLa using 13509-1-AP

p115, USO1 Polyclonal antibody

p115, USO1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.

AmotL2 disrupts apical-basal cell polarity and promotes tumour invasion.

Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.

PMID: 29133483

Defining the proteolytic landscape during enterovirus infection.

Globozoospermia and lack of acrosome formation in GM130-deficient mice.