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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USF1 Monoclonal antibody (67114-1-Ig)

    WB analysis using 67114-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using USF1 Monoclonal antibody (67114-1-Ig)

    WB analysis of HeLa using 67114-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using USF1 Monoclonal antibody (67114-1-Ig)

    WB analysis of HEK-293 using 67114-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using USF1 Monoclonal antibody (67114-1-Ig)

    WB analysis of HT-29 using 67114-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using USF1 Monoclonal antibody (67114-1-Ig)

    WB analysis of K-562 using 67114-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using USF1 Monoclonal antibody (67114-1-Ig)

    WB analysis of HSC-T6 using 67114-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using USF1 Monoclonal antibody (67114-1-Ig)

    WB analysis of NIH/3T3 using 67114-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

USF1 Monoclonal antibody

USF1 Monoclonal Antibody for WB, ELISA
Cat No. 67114-1-Ig
Clone No.1C8F1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, Upstream stimulatory factor 1, USF, USF1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HT-29 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, Jurkat cells, PC-12 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67114-1-Ig targets USF1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17785

Product name: Recombinant human USF1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-310 aa of BC035505

Sequence: MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

相同性解析による交差性が予測される生物種
Full Name upstream transcription factor 1
Calculated molecular weight 34 kDa
Observed molecular weight 38-43 kDa
GenBank accession numberBC035505
Gene Symbol USF1
Gene ID (NCBI) 7391
RRIDAB_2882418
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP22415
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa

Protocols

Product Specific Protocols
WB protocol for USF1 antibody 67114-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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