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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UROS Polyclonal antibody (14913-1-AP)

    WB analysis using 14913-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14913-1-AP (UROS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using UROS Polyclonal antibody (14913-1-AP)

    WB analysis of K-562 using 14913-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14913-1-AP (UROS antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using UROS Polyclonal antibody (14913-1-AP)

    WB analysis of K-562 using 14913-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14913-1-AP (UROS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using UROS Polyclonal antibody (14913-1-AP)

    IP experiment of K-562 using 14913-1-AP

    IP result of anti-UROS (IP:14913-1-AP, 4ug; Detection:14913-1-AP 1:500) with K-562 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using UROS Polyclonal antibody (14913-1-AP)

    IHC staining of human cervical cancer using 14913-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14913-1-AP (UROS antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using UROS Polyclonal antibody (14913-1-AP)

    IHC staining of human cervical cancer using 14913-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14913-1-AP (UROS antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using UROS Polyclonal antibody (14913-1-AP)

    IF Staining of MCF-7 using 14913-1-AP

    Immunofluorescent analysis of MCF-7 cells, using UROS antibody 14913-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

UROS Polyclonal antibody

UROS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14913-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Uroporphyrinogen-III synthase, Uroporphyrinogen-III cosynthase, uroporphyrinogen III synthase, UROIIIS, EC:4.2.1.75

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14913-1-AP targets UROS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6711

Product name: Recombinant human UROS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-265 aa of BC002573

Sequence: MKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLIFTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCGNAEKLAEYICSRESSALPLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNLNSYYSQQGVPASITFFSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVSCTAESPTPQALATGIRKALQPHGCC

相同性解析による交差性が予測される生物種
Full Name uroporphyrinogen III synthase
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC002573
Gene Symbol UROS
Gene ID (NCBI) 7390
RRIDAB_2213824
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP10746
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for UROS antibody 14913-1-APDownload protocol
IHC protocol for UROS antibody 14913-1-APDownload protocol
IP protocol for UROS antibody 14913-1-APDownload protocol
WB protocol for UROS antibody 14913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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